中性粒细细胞免疫.ppt

中性粒细胞 免疫血液学先天性免疫 炎性白细胞粘附级联位点 LFALCAMT ⅥA4VCAM 阝 integr Select Select A signal Chemokines VAYL VAV2. VAY3 PECAMT? Activation Capture Paracellular and transcellular Adhesion trans migratE Arrest nine intraascul Endothelial celis Basement membrane site of inflammation ey et al, Nat hied 2007 炎性白细胞粘附级联位点 Courtesy of Dr. D. vestweber, MUnster 人中性粒细胞抗原(HNA HLA P70-95(?) CD16 NA1. NA2. SH CD11b/CD18 cD177 PECAM-1 JAM- Endothel JAMC介导的不同细胞间的 级联反应 Platelets JAM-C Santoso et a/ J Exp Med 2002 JAM-C eterophilic interaction Dendritic Cells Neutrophils Langer er a, ATVB 2007 Nmmenscnuh et al JLeuk Bo 2009 Chavakis ef a/ JBC 200 Keiper et af FASEB 2005 homophilic interaction 200了 人中性粒细胞抗原( CHNA) Phenotyp HNA Localisation Frequency Frequen Whites Asians NA1 a FCyRIlIb 57-62 88-91% NA2 FCyRIlIb 88-89% 5154% 1c FCyRIlIb 5% 1% HNA-1 null 0.15% 1% NB1 2a CD177 89-99% 98% 5b 3a not known 8996% Mart CD11b Ond CD11a 86-92% Chinese, Japanese, Koreans, or Taiwanese Bux et al. Vox Sang 2008 FCyRIIIb(CD16 上的HNA-1 r936ser AsngESer Asps2Asn NA2 Ig Val1oslle N7 Ala7BAsp SH anchor uuuUUuuuuU ● point O oligosaccharide 嗜中性白细胞抗体的临床影响 非溶血性输血反应发热 输血相关的急性肺损伤门AD 新生儿同种免疫性嗜中性白血球减少症 自身免疫嗜中性白血球减少症 骨髓移植后嗜中性白血球减少症 嗜中性白细胞抗体的实验室诊断 粒细胞免疫荧光试验(GIF 粒细胞凝集试验GA 淋巴细胞免疫荧光试验IFT) 单克隆抗体固定粒细胞抗原法IAGA ELISA 嗜中性粒细胞同种抗体 found in our laboratory (n=31 NA1 HNA-la NA2 HNA-lb SH HNA-1C 75 ND1 HNA-1 NB1 4 HNA-2a 5b HNA-3a 25 5 Mart HNA-4a Ond HNA-5a