判断推理逻辑判断.docVIP

Illumina 测序技术概述 Part #Rev.C ? 2013 Illumina, Inc. All rights reserved. Illumina, IlluminaDx, BaseSpace, BeadArray, BeadXpress, cBot, CSPro, DASL, DesignStudio, Eco, GAIIx, Genetic Energy, Genome Analyzer, GenomeStudio, GoldenGate, HiScan, HiSeq, Infinium, iSelect, MiSeq, Nextera, NuPCR, SeqMonitor, Solexa, TruSeq, TruSight, VeraCode, the pumpkin orange color, and the Genetic Energy streaming bases design are trademarks or registered trademarks of Illumina, Inc. All other brands and names contained herein are the property of their respective owners. 课程目标 课程结束时： 测序流程中的主要步骤清楚了解 Illumina – ）的过程Cluster Generation–能够描述簇生成（ Sequencing by Synthesis）的原理理解边合成边测序（– FOR RESEARCH USE ONLY Part #Rev.C2 Illumina Sequencing Workflow 测序流程Illumina Library Preparation 文库制 cBot Cluster Generation MiSeq 簇生成NextSeq HiSeq 2500-Rapid HiSeq Sequencing HiScan SQ 测序GA IIx MiSeq NextSeq ICS/RTA Data Analysis CASAVA 数据分析MSR BaseSpace FOR RESEARCH USE ONLY Part #Rev.C3 Illumina Sequencing Workflow Illumina 测序流程 Library Preparation 文库制 cBot Cluster Generation MiSeq 簇生成NextSeq HiSeq 2500-Rapid HiSeq Sequencing HiScan SQ 测序GA IIx MiSeq NextSeq ICS/RTA Data Analysis CASAVA 数据分析MSR BaseSpace FOR RESEARCH USE ONLY Part #Rev.C4 文库制备是决定测序实验成功与否的关键步骤 ④ ① ① ③④ ② ② 双端标签文库 ① Flow Cell）结合的区域与流动槽（ 和Read2测序引物结合的区域Read 1② 插入片段 ③ ④ 标签序列区域（Index） 片段两端加上能够与DNA文库制备的目的是在需要测序的 测序仪配合的接头序列（Multiplexed, SR, PE） FOR RESEARCH USE ONLY Part #Rev.C5 Illumina Sequencing Workflow Illumina 测序流程 Library Preparation 文库制 cBot Cluster Generation MiSeq 簇生成NextSeq HiSeq 2500-Rapid HiSeq Sequencing HiScan SQ