毛细管电色谱技术详述.docxVIP

Capillary Electro- Chromatography 毛细管电色谱技术 0.004 AU 10 Time (min) 15 1 = methanol; 2 = resorcinol; 3 = phenol; 4 = p-nitroaniline; 5 = nitrobenzene; 6 = toluene; 7 = 2-naphthol; 8 = Sudan III. Conditions: capillary, 50 urn i.d. 65 cm (effective length 50 cm); run buffer, 30 mM SDS in 50 mM phosphate/100 mM borate (pH 7.0); applied voltage, 15 KV; current, 33 uA; detection, UV absorbance at 210 nm; temperature, 35 ° C? Terabe, Trends Anal. Chem. 8, 129 (1989). Capillary Electro-Chromatography 毛细管电色谱(CEC) Micelle electrokinetic capillary chromatography 胶束电动毛细管色谱(MEKC) Micro emulsion electrokinetic Chromatography 微 乳液电动色谱(MEEKC) Packed capillary electrokinetic chromatography 填充毛细管电动色谱(PCEC) Micelle electrokinetic capillary chromatography- MEKC 胶束电动毛细管色谱法 表面活性剂 ■亲水端和疏水端 ■类型：阴离子、阳离子、两性离子和非离子表面活 性剂 ■胶束的形成 □临界胶束浓度,Critical micelle concentration - CMC Table 4. Surf act ant Classes and Properties Surfactant Type CMC Aggregation # SDS Anionic 8.1 x 2 62 CTAB Cationic 9.2 x 10-4 170 Brij-35 Noni onic 1.0 x 10-4 40 Sulfobetaine Zwitterionic 3.3 x 10 55 SDS = sodium dodecyl sulfate; CTAB = cetyltiiinethylammoniuni bromide; Brij-35 = polyoxyethylene-23-lauryl ether; sulfobetaine = JV-dodecyl-A^JV- dimethyl a mmonhim- 3 ?pTopnne? 1 ? sulfonic acid.  Table 1. Critical Micelle Concentration, Aggregation Number (n), and Kraft point (Kp) of Selected Ionic Surfactants Surfactant CMCa/10 3 M n Sodium dodecyl sulfate (SDS) 8.1 62 16 Sodium tetradecyl sulfate (STS) 2.1 (50°C) 138° 32 Sodium decanesulfonate 40 40 Sodium doclecanesulfate 7.2 54 37.5 Sodium A^-laiiroyl-T^-nietliyltaurate (LMT) 8.7 0 Sodium polyoxyethylene dodecyl ether sulfate 2.8 66 Sodium A^dodecanoyl-Z?valiiiate (SDVal) 5.7 (40°C) Sodium cholate 13-15 2-4 Sodium deoxycholate 4-6 4-10 Soclhini taurocholate 10-15 5 Sodiiun taurodeoxycholate 2-6 Potassium perfluorolieptanoate 28 25.6 Dodecyltrinietliylanimoniuiii chloride (DTAC) 16 (30°C) Dodecyltrinietliylanimoniuiii bromide (DTAB) 15 56 Tetraclecyltrimethylainmonium bromi