（优质医学）二代测序技术在血液系统疾病中的应用浅谈.pptVIP

So far I‘ve been talking about the chemistry of the ngs. They all have similar library preparation to add adapters. The template amplification have two types, one is solid-phase PCR used by illumina, the other is empcr used by 454 and solid. The sequencing step. Illumina and 454 are adapting sbs while solid using sbl. The next step is data analysis which will be presented by chong. * Polony sequencing refers to all commercial technologies except for Helicos. Polony sequencing takes place using array of polonies, in which all amplicons of the same DNA fragment are clustered together on the sa