原发性肝癌组织双向电泳技术优化.docVIP

原发性肝癌组织双向电泳技术优化 　　 　abstract： 【objective】 to establish a set of two-dimensional polyacrylamide gel electrophoresis (2-de) techniques for proteomic research of hepatocellular carcinoma (h) tissues. 【methods】 different methods or condition for key procedures of 2-de were adjusted and optimized， including sample preparation， sample loading methods， iso-electronic focusing (ief)， paring with the gel after massie blue staining. 【results】 high-resolution results with repetitiveness could be obtained aording to the condition： 7 mmol/l urea， 2 mmol/l thisurea， 4%chaps， 100 mmol/l dtt， 0.5% (ph 3-10)