生物化学课件：8 Enzyme.pptVIP

liguofu Many enzymes need non-protein cofactors to help in catalysis For E + S ES E + P Km = If k 2 is rate-limiting, k 2k -1, Km = k -1 /k 1 Km equals to the dissociation constant (Kd) of the ES complex; Km represent a measure of affinity of the enzyme for its substrate in the ES complex. If k 2 k -1, then Km = k 2 / k1 If k2 and k-1 are comparable, Km is a complex function of all three rate constants. The value of kcat/Km has an upper limit (for the perfected enzymes) It can be no greater than k1 The decomposition of ES to E + P c