生物化学课件：5 Amino acids-Peptides-Proteins.pptVIP

Polypeptide chains are cleaved into short fragments by chemical or enzymatic methods and then sequenced by Edman method. Cyanogen bromide (CNBr) cleaves polypeptides on the carboxyl side of methionine residues. A set of proteases cleave peptide chains adjacent to specific amino acid residues: Trypsin, specifically on the carboxyl side of Arg and Lys; Chymotrypsin（胰凝乳蛋白酶）, the carboxyl side of Phe, Tyr, and Trp The fragments produced need to be separated (purified) by chromatographic（色谱分析）or electrophoretic（电泳）methods before they can be sequenced. Large proteins are cleaved into short peptides