影响糖尿病小鼠模型稳定的因素分析.docxVIP

影响糖尿病小鼠模型血糖的因素考察 孙建新，王忠，吕晓敏（新疆医科大学医学实验动物中心，乌鲁木齐 830011) [摘 要 ] 目的：考察四氧嘧啶（ ALX ）诱导法制备糖尿病小鼠模型时影响血糖的因素。方 法：120 只昆明种小鼠随机分为正常组和 5 个剂量的模型组。 正常组小鼠腹腔注射生理盐水； 5 个剂量的模型组小鼠分别腹腔注射 250、 200、150、 100、50 mg/kg 的 ALX ，测量 ALX 的剂量、小鼠的体重、垫料、是否禁食以及禁食时间对小鼠 FBG（空腹血糖）的影响，将 FBG≥ 11.1 mmol/L 的小鼠模型作为建模成功，计算各组小鼠的成模率和死亡率。结果：体 重为 22~28 g 的小鼠腹腔注射 ALX 100~150 mg/kg ，夜间禁食 12~14 h，无垫料的情况下， 可成功构建糖尿病小鼠模型。结论：该方法构建糖尿病小鼠模型成功率高，死亡率低，血 糖稳定。 [关键词 ]  糖尿病模型；影响因素；成模率；死亡率；  FBG [中图分类号  ]  R  [文献标志码  ]  A  [文章编号  ]  1671-2838（ 2011） 04-0000-00 DOI ： 10.5428/pcaInvestigation of influential factors of blood glucose of diabetic mouse models SunJjianXin,WangZHong,LV XiaoMing （ 1.The Medical Laboratory Animal Center of Xinjiang Medical University, Urumqi 830011,China) [ABSTRACT] Objective： To investigate the influential factors of blood glucose on diabetic mice models induced by alloxan. Method: A total of 120 Kunming mice were randomly divided into control group and five model groups. Mice in the control group was peritoneally injected with saline at the dose of 10 ml/kg. Meanwhile, mice in the 5 model groups were peritoneally injected with different doses(250,200,150,100,50 mg/kg) of alloxan, respectively. The influence of doses of alloxan, weight of mice, cushioning materials, breeding environment  ， fasting and fasting time on fasting blood glucose(FBG) were determined. The mice with FBG ≥ 11.1 mmol/L were established as diabetic models. Results: Diabetic mice models can be successfully established using 22~28 g mice, injecting 100~150 mg/kg alloxan, and then fasting 12~14 h in the night and using no cushioning materials. Conclusion: In such conditions, diabetic mice models can be obtained with high success rate, low mortality incidence and stable blood glucose. [KEY WORDS] diabetic models ； influential factors; success rate; mortality incidence;fbg 糖尿病是由多种病因引起的以慢性高血糖为特征的代谢紊乱疾病，伴有因胰岛素分泌和作用缺陷引起的糖、脂肪和蛋白质代谢异常。近年来，该病发病率急剧增加，已经严重危害人类的健康。为了更深入地研究糖尿病的作用机制以及筛选、研发新药物，制备一个 稳定可靠的动物模型至关重要。目前，制备糖尿