血红蛋白诱导的脂肪酸氧化对鲢鱼肌原纤维蛋白氧化的影响以及迷迭香酸抗氧化作用的研究.pdfVIP

Study on the effect of hemoglobin-induced fatty acid oxidation on the silver carp myofibrillar protein oxidation and antioxidant effect of rosmarinic acid Table of Contents 摘要…………………………………………………………………………………………….i Abstract ……………………………………………………………………………………...iii List of Abbreviations ………………………………………………………………vi Chapter 1 Introduction and review of literature 1 1.1 Introduction1 1.2 Literature Review 3 1.2.1 Hemoglobin mediated lipid oxidation 3 1.2.2 Hemoglobin and fatty acids induced protein oxidation4 1.2.3 Inhibition effect of phenolic compounds in hemoglobin induced lipid and protein oxidation 7 1.2.4 Binding interaction between phenolic and proteins 10 1.2 Technical route11 1.4 Objectives 12 Chapter 2 Binding interaction between hemoglobin and fatty acids using spectroscopic measurements 13 2.1 Introduction13 2.2 Materials and Methods13 2.2.1 Chemicals13 2.2.2 Fish supply and bleeding 13 2.2.3 Preparation of Hemoglobin 14 2.2.4 Determination of hemoglobin concentration 14 2.2.5 Fatty acid preparation14 2.2.6 UV-VIS absorption Spectroscopy 15 2.2.7 Fluorescence spectroscopy 15 2.2.8 Circular dichroism spectroscopy 15 2.2.9 Statistical analysis16 2.3 Results and Discussion16 2.3.1 Changes in UV-Vis absorption spectroscopy 16 2.3.2 Fluorescence studies of Hb-fatty acid complex 18 2.3.3 Study of Thermodynamic Parameters 21 2.3.4 Secondary structure estimation by Circular Dichroism (CD)24 2.4 Conclusion26 Chapter