不可培养微生物对环境的作用.docVIP

不可培养微生物对环境的作用 （石河了大学生命科学学院微生物实验室 石河了 832000） 摘要：微生物非培养技术，如DNA指纹技术、焦磷酸测序、分子杂交技术、宋基因组学、宏转录组学、宋 蛋口质组学、代谢组学等的飞跃发展和广泛应用，有助于快速、准确地鉴定白然界或人工生境中微生物种 群，为揭示生物修复过程中微生物群落结构、功能及修复机理提供了重要方法，提高了生物修复技术的检 测效能。通时也为人类观察不可培养的微生物对环境的作用提供了技术基础，如想实时荧光定最PCR、基 因指纹技术、宋基因技术、分子标记技术等。 关键词：不可培养微生物;宏基因组；分子技术;分子标记;基因指纹技术; 目录 TOC \o 1-5 \h \z HYPERLINK [34J Schloss PD, Handelsman J. Biotechnological prospects from melagenomics. Curr Opiu BiotechuoL 2003, 14(3):303-310 Jeon CO, Park W, Padmanabhan P, et al. Discovery of a bacterium with distinctive dioxygenase that is responsible for in situ biodegradation in contaminated sediment. Proc Nall Acad Sci USA, 2(X)3, 100(23): 13591 — 13596 Radajcwski S, Webster q Rcay DS, ct al. Identification of active rncthylotroph populations in an acidic forest soil by Stable-isotope probing. Microbiology, 2002, 148(8):2331 -2342 Galbraith EA. Antonopoulos DA, White BA. Suppressive subtractive hybridisation as a tool for identifying genetic diversity in an environmental metagenome: the rumen as amodcl. Environ Microbiol , 2004,6(9):928-937 Brzostowicz PC, Walters DM, Thomas SM, et al. mRNA differential display in a microbial enrichment culture: Si-multaneous identification of three cyclohexanone monooxygenases from three species? Appl Ernviron Micro-biol, 2003,69( 1 ):334-342 Cramcri R, Suter M. Display of biologically active proteins on the surface of filamentous phages: a cDNA cloning system lor lhe selection of functional gene products linked lo the genetic information responsible lor their production. Gene, 1993, 137(1): 69-75 Demidov VV, Bukanov NO, FrankK-Amcnctskii MD Duplex DNA capture. Curr Issues Mol Biok 2000, 2(1):31-35 Wu L. Thompson DK、Li GS. et aL Development and evaluation of functional gene arrays for the election ofsclcctcd genes in the environment. Appl Environ Microbiol, 2001,67:5780-5790 Zhou J, Bruns MA, Ticdjc JM. DNA recovery from soils of diverse composition. App Microbiol, 1996, 62(2):316-322 Tsai YL, Olson BH. Rapid method for direct ex