大鼠容量超负荷早期心室压力变化和心肌原癌基因c-fos、c-..docxVIP

大鼠容量超负荷早期心室压力变化和心 肌原癌基因 c-fos 、c-jun 、 c-myc、 egr-1 的表达 关键词：心室功能；原癌基因；基因表达 中国病理生理杂志 000705 [ 摘 要] 目的：检测大鼠心脏容量超负荷 (volume overload , VOL时左心室内压变化诱导心肌原癌基因 c-fos、c-jun、 c-myc、egr-1 mRNA表达的时间变化规律。方法：测定 VOL、假手术对照组大 鼠术后30 min、1、4、6、12及48 h左心室收缩压(LVSP)和舒张末压 (LVEDP)，用狭缝杂交检测2组各时间左室心肌原癌基因表达，用密度仪进行定 量分析。结果:VOL大鼠术后LVSP显著低于假手术组(PvO.05) , 48 h最低；术 后30 min LVEDP高于假手术组(P0.05) , 12 h最高，48 h与假手术组相近 (P0.05);假手术组和阴性对照各原癌基因无阳性表达， AVF后 1 h检测到c- fos、c-jun、egr-1 mRNA表达，4 h检测到c-myc表达，均于4 h出现表达峰 值，此后减弱，48 h c-fos无表达，c-jun表达较弱，c-myc和egr-1仍有较 高表达。结论：VOL大鼠LVEDP曾加，首先诱导c-fos、c-jun、egr-1表达， c-myc 表达较晚， c-myc、 c-jun 、 egr-1 表达持续时间较长，可能反映了活体 VOL刺激心肌原癌基因表达时间变化规律；当室壁受到一定程度和一定时间的 负荷牵拉后心肌原癌基因即开始表达，负荷的强弱可能并非重要因素。 [中分类号] Q754； K346.1 [ 文献标识码 ] A [ 文章编号 ] 1000-4718(2000)07-0592-04 Correlation between intraventricular pressure and early phase proto-oncogene expression in volume overload rats HU Xiao-ping (Department of Pathology, Punan Hospital, Shanghai 200125, China) LI He ， ZHANG Guo-yua，n WU Zong-gui (Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China) FEI Jian (Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031, China) [Abstract] AIM: To investigate the effect of intraventricular pressure change by volume overload (VOL) on expression of protooncogene c-fos,c-jun,c-myc and egr-1. METHODS: Left ventricular systolic pressure(LVSP) and left ventricular end diastolic pressure (LVEDP) of rat with VOL induced by aortacaval fistula operation and rats of control group were measured at 30 min, 1, 4, 6, 12 and 48 h after the operation,these mRNAs at the foregoing time points were measured by slot bloting method and quantified with densitometry. RESULTS: Be compared with the control group, VOL rats LVSP decreased (P0.05) and declined most remarkably at 48 h,LVEDP elevated significantly at 30 min (P0.05), reached a maximal value at 12 h and the levels of control group at 48 h (P0.05) after the operation.The proto-oncogene expression