第8章病毒的遗传分析.ppt

测验步骤： amA ＋ tsC × ＋amB ＋ su- 许可条件 野生型重组子 ++tsC、++tsC+ 限制条件 tsC和tsC+所占比例，确定基因排列顺序 总数 tsC+ amA + tsC + amB + + + tsC + + + tsC amA + + + amB + + + tsC + + 正交 顺序I 顺序II 多 少 多 少 amA + + + amB tsC + + + + + tsC + amA + tsC + amB tsC + + + + + 反交 顺序II 顺序I 多 少 多 少 如果tsC+占优势，则为顺序tsCamAamB; tsC占优势则为amAamBtsC 四、Benzer的重组测验 New York. parents emigrated from Warsaw, Poland and worked in the garment industry(dissected flies in his basement lab and read books on atomic physics during synagogue(教堂)) Benzer returned to Purdue as a professor of physics, but spent most of his time traveling to other labs to work in molecular biology. Benzer took the summer bacteriophage course at C.S.H.L in 1948 with Gunther Stent. After struggling to learn the courses most important skill – holding a test tube in one hand and the test tube cap and pipette in the other – Benzer was hooked on biology. Benzer read a short book called What is Life?. Erwin Schrodingers book had a similar effect on Benzer because it made the mysterious nature of genes sound like the problem to solve. At 15, Benzer graduated from physics and chemistry at Brooklyn College, and continued study physics at Purdue University, worked on radar project. Seymour Benzer(1921- In 1953, after model of DNA published, Benzer hatched his plan to get inside the gene by using bacteriophage with mutant rII genes. Max Delbrück ridiculed the plan and told Benzer you must have drunk a triple highball before writing this. Benzers 5-year-old daughter Martha liked the plan better and sketched her vision of two phages infecting a bacterium. In 1971, Benzer received the Lasker Award for this brilliant contribution to molecular genetics. After ten years of work on the rII system, and prompted by observations of his two daughters, Benzer began studying how genes shape behavior. As a professor of biology at Caltech, he and his graduate student Ronald Konopka were the f