过氧化物酶体增殖物激活受体―γ激动剂对急性胰腺炎大鼠核转录因子―κBToll样受体4通路的调节及肾损伤的保.docxVIP

过氧化物酶体增殖物激活受体― γ激动剂对急性胰腺炎大 鼠核转录因子― κBToll样受体 4 通路的调节及肾损伤的保护 [摘要  ]  目的 探讨过氧化物酶体增殖物激活受体  -γ 激 动剂对急性胰腺炎大鼠核转录因子  -κ B/Toll  样受体  4 通路的 调节以及肾损伤的保护作用。  方法 选择  72 只  SD 大鼠随机 分为假手术组 （ SO组）、模型组（ SAP组）和罗格列酮组 （ROSI 组），各组再随机分为术后 6 h、 12 h、 24 h?M，每组 8 只。 SAP组采用两次腹腔注射 L-精氨酸制备 SAP模型，ROSI组 30 min 后股静脉注射 10%罗格列酮溶液 6 mg/kg ， SO组腹腔注 射等体积生理盐水。 观察大鼠肾脏病理改变， 测定肾脏 TLR4、 NF-κB 蛋白表达水平， 检测血清 TNF-α 、IL-1β 、IL-6、BUN、 Cr 含量。 结果 ROSI组大鼠肾小管细胞病理损害较 SAP组减轻；与 SAP组比较，肾组织内 NF-κ B、TLR4蛋白明显下降 P [关键词 ] 急性胰腺炎；过氧化物酶体增殖物激活受体；核转录因子 -κB； Toll 样受体 [中图分类号 ] R576 [文献标识码 ] A [文章编号 ] 1673-9701（ 2016） 34-0031-04 Regulation of nuclear factor- κ B/Toll-like receptor 4 pathway and protection of renal injury in rats with acute pancreatitis by peroxisome proliferator activated receptor- γ agonist LU Bei1 YU Yuanquan2 YIN Junjie1 CAI Yang1 1.Department of Hepatobiliary Surgery ， Hangzhou First Peoples Hospital， Hangzhou 310006， China； 2.Department of Hepatobiliary Surgery ， the Second Affiliated Hospital of Zhejiang University School of Medicine ， Hangzhou 310009， China [Abstract] Objective To investigate the regulation of nuclear factor- κB/toll-like receptor 4 pathway and protection of renal injury in rats with acute pancreatitis by peroxisome proliferator activated receptor- γ agonist. Methods 72 SD rats were randomly divided into sham operation group （SO group）， model group（SAP group） and rosiglitazone group（ROSI group）. The rats in each group were further randomly divided into postoperative 6 h ， 12 h and 24 h group ， with 8 rats in each group. In the SAP group， SAP model was prepared by intraperitoneal injection of L-arginine twice. In ROSI group ， 6 mg/kg of 10% rosiglitazone solution was intravenously injected after 30 min. In the SO group ， equal volume of normal saline was injected intraperitoneally. The pathological changes of rats kidney was observed. The expression levels of TLR4 and NF-κ B in kidneys were measured. The levels of TNF-α ， IL-1β ， IL-6， BUN and Cr in serum were measured. Results T