化学生物学中的探针标记特征技术.pptVIP

;;;Target probe generation;;;Science, 312, 217-224 (2006).;Science, 312, 217-224 (2006).;;;;;绿色荧光蛋白GFP的最初发现者;GFP作为发光的遗传标签;2008年诺贝尔化学奖揭晓;;钱永健的贡献;钱永健的第二项工作是GFP。1994年起，钱永健开始研究GFP，改进GFP的发光强度，发光颜色（发明变种，多种不同颜色），发明更多应用方法，阐明发光原理。世界上应用的FP，多半是他发明的变种。他的专利有很多人用，有公司销售。 钱永健的工作，从八十年代一开始就引人瞩目。他可能是世界上被邀请给学术报告最多的科学家，因为化学和生物界都爱听他的报告，既有技术应用、也有一些很有趣的现象。他1952年出生，年龄允许他等些年（而下村修没有这个优势）。所以，很多人多年认为钱永健会得诺贝尔奖，可以是化学、也可以是生理奖。值得指出，钱永健非常肯定下村修的工作，钱较早公开介绍下村修的发现。;A guide to choosing fluorescent proteins;钱永健;;二十种标准氨基酸;;;;;;;;;;;;;;;;LN Wu, XM Yan*, et al. Anal. Chem., 2014, 86, 907–912.;;;将Jurkat细胞放在含叠氮乙酰甘露糖胺的培养液中生长，叠氮基团可以通过唾液酸生物合成途径引入到细胞表面的糖蛋白上。此细胞和生物素化的膦作用，细胞上的叠氮基和膦反应生成一个稳定的肽键，从而形成一个非天然的生物素化的细胞表面，加入连有抗生物素的荧光探针，就实现了细胞表面的荧光标记。;The delivery of azides to cell surfaces through other carbohydrate biosynthetic pathways could significantly expand applications of cell surface engineering. Azides and phosphines are abiotic structures both inside and outside cells, which raises the exciting possibility that their ligation could proceed in the intracellular environment. Given existing powerful methods for incorporating unnatural building blocks into other biopolymers, one need not be restricted to cell surface oligosaccharides as hosts for these chemical handles. Azido–amino acids, for example, could be introduced into proteins and later targeted with phosphine probes. ;Saxon E, Bertozzi CR. Science, 287, 2007-2010 (2000).;Distinguished Professor of Chemistry Professor of Molecular and Cell Biology at UC Berkeley. B.S. Chemistry, Harvard University,1988 Ph.D. Chemistry, UC Berkeley, 1993 Postdoc Cellular immunology, UCSF, 1993-1996 Faculty, UC Berkeley, 1996 Elected member of the American Academy of Arts and Sciences, 2003 Elected member of the National Academy of Sciences, 2005 Prof. Bertozzi’s research interests span the disciplines of chemistry and biology with an emphasis on studies of cell surface glycosylation pertinent to disease states. Her lab focuses on profiling changes