第四节ecoli半乳糖操纵子.ppt

第四节 E coli半乳糖操纵子;;The E. coli galactose operon.;第四节Ecoli半乳糖操纵子;The DNA sequence of the regulatory region of the E.coli gal operon (A) and that of the pLacZ plasmid used for construction of the artificial gal operator promoter derivatives (B). The Oi and Oe sites of the gal operon are in pink lettering, as are the XbaI and SpeI restriction sites of pLacZ for introduction of the Oi and Oe operators, respectively. The cAMP–CRP site is in blue lettering. HU binds to the underlined sequence. The ‘Pribnow boxes’ of the promoters are framed in yellow. The coding sequences for β-galactosidase and epimerase are framed in green. From “A functional assay in Escherichia coli to detect non-assisted interaction between galactose repressor dimers ” Nucleic Acids Research ;The cAMP–CRP complex abolishes transcription from P2 and is required for P1 transcription.;P1 启动的转录需要cAMP–CRP，在葡萄糖不存在时才能进行； P2启动的转录当有葡萄糖（无cAMP–CRP）时才能进行； ;半乳糖的双重作用;只要无半乳糖，无论有无葡萄糖，gal操纵子都开放;Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon Genes Dev. July 1, 2002 16: 1696-1706;Thorleif M?ller et al. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark;第五节 阿拉伯糖操纵子;第四节Ecoli半乳糖操纵子;第四节Ecoli半乳糖操纵子; araC基因的编码产物AraC为一同源二聚体，它与araO1结合，强烈阻遏araC基因的表达，形成典型的反馈抑制系统。 当阿拉伯糖存在时，AraC与之结合，虽仍能阻遏araC基因的转录，但强度有所减弱。结合型和游离型AraC对araC的阻遏作用位点均在araO1区域内，由于它与启动子ParaC相互重叠，所以其阻遏作用的机制是妨碍RNA聚合酶与启动子ParaC的相互作用。 ;第四节Ecoli半乳糖操纵子;第四节Ecoli半乳糖操纵子;第四节Ecoli半乳糖操纵子;Dual positive and negative control: the arabinose operon Dual control of the ara operon. (a) In the presence of arabinose, the AraC protein binds to the araI region and, when bound to cAMP, the CAP protein binds to a site adjacent to araI. This binding stimulates the transcription of the araB, araA, and araD genes. (b) In the absence of arabinose, the AraC protein binds to both the araI and araO regions, forming a DNA loop. This binding prevents transcription of the ara operon. ; Figu