植物种质保存技术和人工种子在农业方面实际应用.pptVIP

第五章 植物种质的保存技术和人工种子在农业方面的应用;;第一节 植物种质的保存;;离体种质保存;;一、继代培养保存;二、限制生长保存;二、限制生长保存;;三、超低温保存;三、超低温保存;;;;;（二）、玻璃化保存方法;;;第二节　人工种子的制备;一、人工种子的概念;根据包被的需要程度可分为三类：;;根据包被的需要程度可分为三类：;根据包被的需要程度可分为三类：;;;与试管苗技术和自然有性繁殖种子相比的优点：;与试管苗技术和自然有性繁殖种子相比的优点：;二、繁殖体的类型及其生产;体细胞胚的定义;;二、繁殖体的类型及其生产;二、繁殖体的类型及其生产;;（二）、繁殖体的生产;三、人工种子包被;（二）、繁殖体的包埋;海藻酸钠作包埋介质的操作程序;;;;（三）、人工种皮的研制;;（四）、人工种子的发展、应用前景、问题;（四）、人工种子的发展、应用前景、问题;（四）、人工种子的发展、应用前景、问题;;第三节 植物的快速繁殖; 一、植物快速繁殖的类型;;;;;;;;;;;;;;;;;Fig Establishment of shoot cultures and propagation of shoots by different bioreactors. B： A shoot cluster produced 8 weeks following crown-tip meristem isolation. C： Air-lift bioreactor after a 10-week culture of 200 shoot clusters. D：Rotating bioreactor after an 8-week culture of 30 shoot clusters. E：Periodic immersion bioreactor (PIB) after an 8-week culture of 150 shoot clusters. F：A shoot cluster produced by PIB. G： Shoots of different sizes (2–6 cm) separated from a cluster. H： Shoots in a tray containing rooting medium. I： Plants after transfer to soil; Fig. A–D Methodology of bioreactor-based micropropagation. Drawing schematic illustration of PIB. A：Shoot multiplication by longitudinal section preparation. B： Comparison of PIB (left) and air-lift bioreactor (right) for mass of shoots produced in 6 weeks. Equal numbers of shoots were placed in each bioreactor. C：Production of healthy shoots in PIB. D：A pineapple plant, produced via PIB, grown in the field in Costa Rica showing normal leaves. ;;;;;二、炼苗和移栽;;;;;;;;;