基因组装技术在合成生物学中的应用.docx

微生物学通报 Jun. 20, 2018, 45(6): 1358- 1368 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.170548 专论与综述 基因组装技术在合成生物学中的应用 黄鹏伟1 龚大春 2 戴传超 1 梅艳珍 1* (1. 南京师范大学生命科学学院 江苏省微生物与功能基因组学重点实验室 江苏省微生物资源产业化工程技 术研究中心 江苏 南京 210023) (2. 三峡大学生物与制药学院 湖北省天然产物研究与利用重点实验室 湖北 宜昌 443002) 摘 要： 基因组装技术是合成生物学领域近年来发展起来的新型技术。它基于大规模基因组数据分析，发现新型的或隐藏的生物活性物质合成基因簇。利用基因组装技术，可提高或激活沉默的生物合成基因簇在微生物中的表达，从而合成潜在的、有价值的生物活性物质。本文旨在阐明最新的体内和体外基因组装技术的设计原理、关键策略及其应用。基因组装技术是合成生物学、代谢工程和功能基因组学研究的重要工具，对生物活性物质的高效生产及合成具有重要意义。 关键词： 基因组装， OE-PCR ，同源重组，酿酒酵母 ( Saccharomyces cerevisiae)，合成生物学 211*Application of gene assemblies in synthetic biology 2 1 1* 1HUANG Peng-Wei 1 GONG Da-Chun DAI Chuan-Chao MEI Yan-Zhen (1. Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China) (2. Hubei Key Laboratory of Natural Products Research Development, College of Biological Pharmaceutical, China Three Gorges University, Yichang, Hubei 443002, China) Abstract: Gene assemblies are new technologies developed in recent years in the field of synthetic biology. They are based on large-scale analysis of genome sequences to find new or cryptic gene clusters which synthesize bioactive substances. The silent or cryptic gene clusters in microorganisms can be activated to synthesize potential valuable compounds by these technologies. We aim to elucidate the principle, the key strategy and the application of in vivo and in vitro gene assemblies. These technologies play an important role in synthetic biology, metabolic engineering and functional genomics and they will be of great value to efficiently produce bioactive compounds. Keywords: Gene assemblies, OE-PCR, Homologous recombination, Saccharomyces cerevisiae , Synthetic biology Foundation item: National Natural Science Foundation of China * Corresponding author: E-mail: meiya