动脉内皮损伤后平滑肌细胞维甲酸受体的表达及维甲酸的作用解读.docx

动脉内皮损伤后平滑肌细胞维甲酸受体 的 表达及维甲酸的作用 * 團 摘 要 目的：研究动脉内皮损伤后 VSMC 中 RAF 表达的规律及其与内 膜增生 的关系。方法：放射配基法测量用维甲酸与未用药两组内皮损伤后不同 时期的受体最大 结合容量和平衡解离常数。同时用形态学方法测量血管内膜面 积。结果：动脉内皮损伤 后早期 VSMC 中 RAR 数量显著低于正常 ( Pv0.01) ，于 面积增加，用维甲酸后， 后 2 周即接近正常，血管内膜面积显著少于未用药组。 第 4 周恢复正常，同时血管内膜 RAR 显著增加，于术 结论：动脉损伤后早期 RAR 表达下调，维甲酸能向上调节其表达， RAR 可能在动脉内皮损伤后的内膜增 生中 起重要作用。 主题词 动脉粥样硬化；维甲酸；家兔 Retinoic acid receptor expression in arterial smooth muscle cells after endothelial injury and effects of all -trans retinoic acid LI Da-Zhu, CAO Lin-Sheng, FENG Yi-Bai, ZHEN Qiu-Tang, LI Yu-Shu, MAO Yi, SONG Ji n-Xin In stitute of Cardiovascular Disease, Tongji Medical Un iversity, Wuhan (430022) Abstract AIM: To investigate expression of retinoic acid receptor (RAR) in smooth muscle cells after endothelial injury and its relation to intimal hyperplasia. METHODS : Rabbits were divided into two groups:all-trans retinoic acid (RA) treated group and non-drug group, arteries were harvested 24h, 1,2 and 4 weeks after injury. Receptor Bmax and K D were calculated by the use of [ 3 H ] -RA radioligand binding method, neointimal area(IA) were measured morphologically RESULTS : Compared with no rmal arteries, nu mber of RAR in smooth muscle cells reduced significantly (P v 0.01) in the first two weeks after injury, and recovered to normal in the 4th week, at the mean time, IA in creased sig nifica ntly .In RA treated group, RAR nu mber in creased sig nifica ntly whe n compared with non-drug group, and were about to no rmal level 2 weeks after injury. IA decreased at the same time. CONCLUSION : RAR down regulation existed in the early stage after endothelial injury, RA upregulated RAR expressi on, RAR might play an importa nt role in in timal hyperplasia after artery injury. MeSH Atherosclerosis; Tretio nin; Rabbits 血管平滑肌细胞 (vascular smooth muscle cells, VSMC) 的迁移增殖是许 多增生性血管疾病发生发展的重要环节⑴， 而迁移增殖的 VSM