葎草多糖超声提取及抑菌活性探究.docVIP

律草多糖超声提取及抑菌活性探究 【摘要】 目的优化律草多糖的超声辅助提取工艺, 并研究其抑菌活性。方法以多糖中的葡萄糖含量为指标，对 超声波功率、料液比和超声时间进行L9 (34)正交实验，确 定超声辅助提取律草粗多糖的最佳工艺。用DE-52交换柱对 律草粗多糖进行分离，以滤纸片法研究律草粗多糖和多糖分 离组分的抑菌能力。结果以水为提取溶剂，料液比1：28 (g/ml),超声功率120W,超声90 min提取2次，律草多糖 的得率为0.755%。律草粗多糖的抑菌效果具有一定的剂量 依赖性；各多糖组分中HSP-I、HSP-IIL HSP-IV发挥主要抑 菌作用，其中HSP-I效果最显著，HSP-II抑菌效果不明显， 且各组分间表现出协同关系。结论超声波辅助提取摩草多糖 方法可行，得率高。律草多糖具有抑菌活性。 【关键词】律草多糖分离抑菌活性 Abstract : ObjectiveTo optimize the ultrasound-assisted extraction method and to study the antibacterial activity of polysaccharides from Humulus scandens. MethodsTaking the content of glucose as observation index, we optimized the extraction process by L9 (34) orthogonal experiment. The Humulus scandens polysaccharides (HSP) was purified into HSP- I , HSP-II, HSP-III and HSP-IV by ion exchange chroma to graphy with DE-52. The antibacterial activity of each component was tested by means of Iter paper. ResultsThe optimal extraction conditions were obtained as follows： water refluxing, a ratio of solid -liquid as lg： 28ml, 120W for the intension of ultrasonic power, extracting time 90min and extracting two times. Under aforesaid optimization conditions, the yield of polysaccharides was 0. 755%. Antibacterial experiments indicated that the inhibitory effect of crude polysaccharides showed dose-dependent. Among the isolated components, HSP- I , HSP-III, HSP-IV played main antibacterial roles and HSP- II was ineffective. It was coopera tive with different isolated components. ConclusionThe yield of Humulus Scandens polysaccharides is high with ultrasound-assisted extraction method. The Humulus scandens polysaccharides has obvious antibacterial actio n. Key words: Humulus scandens； Polysaccharides； Isolation； Antibacterial activity 律草Humulus scandens (Lour?)Merr.又名拉拉藤，拉拉秧，一年生或多年生草本植物，全国各地几乎均有分布。# 草生长旺盛，富含营养，既可以作为饲料，提高饲喂动物的 生长状况，又可以药用，有清热解毒、利尿消肿之功效［1］。 据文献报道，律草中含有木犀草素、葡萄糖昔、胆碱、挥发 油、软质、树脂、天门冬酰胺等物质［2］,其提取物具有抗 结核菌的功效［3］,因而渐渐引起人们的研究兴趣。近年对 于牽草中的木犀草素、黄酮类物质的提取已有少量研究［4, 5］,但对摩草中多糖类物质的提取和活性研究还未见报道。 本文采用超声波辅助提取方法制