蝉拟青霉不同纯化组分对体外抗肿瘤作用基础探究.docVIP

蝉拟青霉不同纯化组分对体外抗肿瘤作用基础探究 摘要：目的：为了进一步研究蝉拟青霉总多糖和腺昔单用 或分别联合顺钳(DDP)或阿霉素(ADM)在体外的抗肿瘤作用。 方法:用MTT法检测DDP、ADM、总多糖、总腺昔单独及联合 用药对人肺腺癌(PAA-1)细胞的生长抑制能力；用流式细胞 仪检测其对体外培养细胞系细胞周期的影响；用光镜检查其 对体外培养PAA-1细胞系集落形成率的影响；用流式细胞仪 检测其对体外培养淋巴细胞亚群分化的影响。结果:DDP、 ADM、总多糖、腺昔对人肺腺癌(PAA-1)细胞生长抑制的IC50 分别为 9. 05 u g/mL、36. 21 u g/mL 10. 86mg/mL、7. 81mg/mLo DDP或ADM和多糖联用对PAA-1细胞的生长抑制主要表现出 拮抗效应；0. 5mg/mL、0. 25mg/mL腺昔与4 u g/mL阿霉素联合 作用时，显示出拮抗效应,而当8mg/mL腺昔与4 u g/mL阿霉 素联用时，显示协同效应；不同浓度腺昔单用与和DDP联合使 用相比，对细胞生长影响无显著性差异;腺昔和ADM联合作用, 随着腺昔浓度的增加,GO/G1期细胞逐渐减少，而G2/M期相对 增加。对体外培养PAA-1细胞系集落形成率多糖组和阴性对 照组未见明显差异，不同浓度腺昔组的集落形成率略低于阴 性对照组，随着腺昔浓度逐渐增加，集落形成率逐渐下降。多 糖对体外培养淋巴细胞亚群的分化无影响。结论：总多糖、 腺昔具有一定的体外抗肿瘤作用，总多糖与DDP或ADM联用 不能增加其体外的抗肿瘤作用，腺昔与ADM联用可增加其体 外的抗肿瘤作用。 关键词：蝉拟青霉；总多糖;腺昔;肿瘤；体外? 中图分类号:R285.5文献标识码：A文章编 号：1673-7717 (2010)04-0760-05 Research of Anti-tumor Effects of Different Purified Components of Cordyceps Cicadae in Vitro CAI Ju?fen, JIANG Zhi^ming, LU Hong^yang, LU Bo?zheng (Zhejiang Cancer Hospital, Hangzhou 310022, Zhejiang, China) Abstract：?Objective：? To further study anti-tumor effect in vitro of polysaccharides and adenosine respectively, or combined with cisplatin (DDP) or adriamycin (ADM)? ?Methods：? Detection of inhibition of human lung adenocarcinoma (PAAT) cell growth with DDP, ADM, polysaccharides and adenosine respectively or combined administration by MTT assay, cell cycle by f 1 ow cytometry on cultured cell lines, colony-fonning rate of PAA-1 cell in vitro by light microscope examination, and lymphocyte subsets by f 1 ow cytometry. ? Results： ? IC50 of DDP, ADM, total polysaccharides, and adenosine on growth inhibition of human lung adenocarcinoma (PAA-1) cell were 9. 05 u g/mL, 36.21 P g/mL, 10?86mg/mL and 7.81mg/mL. It is antagonist of Polysaccharide combined with ADM or DDP on growth inhibition of PAA-1 cells? Adenosine with 8mg/mL combined with ADM with 4 u g/mL indicate synergisnie It is no different between different concentrations of adenosine alone and combined with DDP on cell growth .With the increase of concentr