袁俊杰 文献简报21.5.16(1)(2022年-2023年).pdfVIP

袁俊杰 文献简报 2021-05-16 A novel lncRNA SOX2OTpromotesthe malignancy of human colorectal cancerby interactingwith miR-194-5p/SOX5 axis YeFeng,YingXu,YongjianGao,YiyingChen,XuefengWangZhi Chen Nature DOI:10.1038/s41419-021-03756-y ABSTRACT: Long noncoding RNAs (lncRNAs) show emerging roles in colorectal cancer (CRC) development and are considered to be involved inthepotentialmechanism oftumormalignancy.While Sox2 overlapping transcript (SOX2OT) has been implicated in the progression of multiple cancers, its role in CRC remains to be explored. In this study, in situ hybridization (ISH) and qRT-PCR were performed to establish the functional relationships between SOX2OT and CRC deranged in CRC tissue and cells. Subsequently, SOX2OT shRNAs vectors were transfected into CRC cells to performed loss-of-function assays to detect thepotentialrole of SOX2OT on proliferation and metastasis in vitro and vivo. The results showed SOX2OT was an oncogene that was up-regulated in human CRC tissues and cell lines. SOX2OT silencing suppressed cell proliferation, migration, and invasion in CRC cells in vitro, and inhibited tumorigenesis in themouse xenografts.Bioinformatic 袁俊杰 文献简报 2021-05-16 predictive analysis coupled with the dual-luciferase reporter, RNA immunoprecipitation (RIP), and functional rescue assay elucidated the mechanistic network of the SOX2OT-miR-194-5p-SOX5 axis in CRC. Mechanistically, SOX2OT acted as a competing endogenous RNA (ceRNA) to upregulate SOX5 by sponging miR-194-5p. Downregulated SOX2OT boosted miR-194-5p expression, thus decreased the protein levelof SOX5,which suppressestumorgenesisofCRC. RNA lncRNA CRC 长非编码 （ ）在结直肠癌 （ ）的发展中显示出 新兴的作用，并被认为与肿瘤恶性肿瘤的潜在机制有关。尽管S