袁俊杰 文献简报21.6.11(2022年-2023年).pdfVIP

袁俊杰 文献简报 2021-06-13 Diagnosis andprognosis of myocardial infarction on aplasmonic chip WeiXu,LinWang,RuZhang,Xuming Sun,LinHuang,HaiyangSu,Xunbin Wei,Chia-Chun Chen,JiataoLou,HongjieDai Kun Qian Nature DOI:10.1038/s41467-020-15487-3 ABSTRACT: Cardiovascular diseases lead to 31.5% of deaths globally, and particularly myocardial infarction (MI) results in 7.4 million deaths per year. Diagnosis of MI and monitoring for prognostic use are critical for clinical management and biomedical research, which require advanced tools with accuracy and speed. Herein, we developed a plasmonic gold nano-island (pGold) chip assay for diagnosis and monitoring of MI. On-chip microarray analysisofserumbiomarkers(e.g.,cardiactroponin I) afforded up to 130-fold enhancement of near-infrared fluorescence for ultra-sensitive and quantitative detection within controlled periods, using 10  μL of serum only. The pGold chip assay achieved MI diagnostic sensitivity of 100% and specificity of 95.54%, superior to the standard chemiluminescence immunoassay in cardiovascular clinics. Further, we monitored biomarker concentrations regarding percutaneous coronary intervention for prognostic purpose. Our work demonstrated a designed approach using plasmonic materials for enhanced diagnosis and 袁俊杰 文献简报 2021-06-13 monitoringforprognosticusetowardspoint-of-caretesting. 心血管疾病导致全球 31.5% 的死亡，尤其是心肌梗塞 (MI)，每 740 MI 年导致 万人死亡。 的诊断和预后监测对于临床管理和生物 医学研究至关重要，这需要具有准确性和速度的先进工具。在此，我 们开发了一种用于 MI 诊断和监测的等离子体金纳米岛 (pGold) 芯 片分析。血清生物标志物 （例如心肌肌钙蛋白 ）的片上微阵列分析I 使近红外荧光增强了 130 倍，用于在受控时间内进行超灵敏和定量 检测，仅使用 10 μL 血清。pGold 芯片检测实现了 100% 的 MI 诊 断灵敏度和 95.54% 的特异性，优于心血管诊所的标准化学发光免疫 检测。更多，我们监测了有关经皮冠状动脉介入治疗的生物标志物浓 度，以用于预后目的。我们的工作展示了一种使用等离子体材料的设 计方法，用于增强诊断和