珠子参水提物抗炎镇痛作用实验研究.docVIP

PAGE PAGE 5 珠子参水提物抗炎镇痛作用实验研究 [通信作者] 贺海波,电话：（0717）6396558, E-mail: shmq0212@126.com. 贺海波*, 1, 石孟琼#, 2, 陈涛*, 1, 卢训丛*, 1, 覃宁玲#, 2, 陈述威#2（443002湖北 宜昌，*三峡大学医学院：中药药理实验室1，#三峡大学医学院基础医学实验中心2） [摘要] 目的：观察珠子参水提物的抗炎、镇痛作用。方法：将300只小鼠随机分成5组，每组10只，分别进行抗炎和镇痛系列实验，其中3组分别给予珠子参水提物低（2.50 g/kg）、中（5.00 g/kg）、高剂量（10.00 g/kg）及阿司匹林(0.20 g/kg)灌胃，空白对照组给予相同体积蒸馏水惯灌胃。采用二甲苯致小鼠耳廓肿胀法、角叉菜胶致小鼠足跖肿胀法和小鼠棉球肉芽肿法观察珠子参水提物的抗炎作用，采用醋酸扭体法和热板法观察其镇痛作用。结果：与空白对照组比较，珠子参水提物珠子参水提物低、中、高剂量组均可明显抑制二甲苯所致小鼠耳廓的肿胀度，抑制角叉菜胶所致小鼠足肿胀和小鼠棉球肉芽肿；可延缓醋酸所致小鼠扭体的出现时间，减少扭体反应次数，提高热板所致小鼠痛阈值(P [关键词] 珠子参；抗炎；镇痛 Anti-inflammatory and analgesic effect of water extractive of Rhizoma Panacis Majoria He Haibo1, Shi mengqiong2, Chen Tao1, Lu Xuncong1, Qin Ningling2, Chen Shuwei2 (1Laboratary of Pharmacology on Traditional Chinese Medicine, 2Experimental center of medical foundation Medical Science College, China Three Gorges University, Yichang Hubei, 443002) [Abstract]: Objective: To study anti-inflammatory and analgesic actions of Rhizoma Panacis Majoria. Methods: 300 mice were randomly divided in five groups, a set of 10 animals, group 1 was given distilled water as control group, group 2 was given low-dose (2.50g/kg) Rhizoma Panacis Majoria, group 3 was given middle-dose (5.00g/kg) Rhizoma Panacis Majoria, group 4 was given high-dose (10.00g/kg) Rhizoma Panacis Majoria, group 5 was given aspirin (0.20 g/kg). Anti-inflammatory effects of Rhizoma Panacis Majoria were investigated through xylene-induced auricle tumefaction, carrageenan-induced hind paws swelling and cotton-induced granuloma in mice; Analgesic effects of Rhizoma Panacis Majoria were investigated through acetic acid writhing test and hot plate test in mice. Results: Compared with control group, the water extractive three different dose groups of Rhizoma Panacis Majoria were able to significantly inhibit the mice auricle tumefaction induced by xylene, hind paws swelling induced by carrageenan and granuloma induced by cotton; The pains in mice caused by acetic acid and hot plate, s