血必净对大鼠急性胰腺炎保护作用机制探讨概要.docVIP

血必净对大鼠急性胰腺炎保护作用机制探讨概要 血必净对大鼠急性胰腺炎保护作用机制探讨概要 PAGE / NUMPAGES 血必净对大鼠急性胰腺炎保护作用机制探讨概要 血必净对大鼠急性胰腺炎保护作用机制探讨 辑： studa20 [ 10-10-30 16:20:00 ] 作者：张云民 编 【摘要】 目的 探讨血必净对大鼠急性胰腺炎的保护作用及机制。方法 SD大鼠 48 只, 随机分为假手术组、胰腺炎组和治疗组，每组再分为 4 h 、8 h 亚组。假手术组仅行开关腹手术，胰腺炎组以牛磺胆酸钠造模，治疗组以血必净干预。光镜观察胰腺组织病理改变，免疫组化法测胰腺组织肿瘤坏死因子 (TNF)- α 、干扰素 (IL)-1 β。结果 治疗组胰腺组织损伤较胰腺炎组明显减轻， 治疗组胰腺组织中 TNF-α、IL-1 β 含量显著低于胰腺炎组 (P0.01) 。结论 血必净可能通过抑制 TNF-α、IL-1 β 的产生减轻胰腺炎症。 【关键词】 血必净 ; 胰腺炎 ; 肿瘤坏死因子 ; 干扰素 Abstract ： Objective To investigate the protective effect of Xuebijing (XBJ) on acute pancreatitis in rats and its possible mechanism. Methods 48 SD rats were randomized into 3 groups: sham group, pancreatitis group and XBJ group, each group sub-divided into 4h and 8h subgroups. In the sham groups, the rats only underwent opening and closure of the abdomen; the pancreatitis groups received taurocholate sodium administration to establish animal model and the treatment groups were intervened by Xuebijing. The pathologic changes of pancreatic tissues were observed by optic microscopy. The expressions of tumor necrosis factor (TNF)- α and interleukin (IL)- 1β in pancreatic tissues were determined by immunohistochemical methods. Results The histomorphological changes of the pancreatic tissues in XBJ group were much more relieved than those in the pancreatitis group. The expressions of TNF- α and IL-1 β were significantly lower in XBJ group than those in the pancreatitis group (P0.01). Conclusion XBJ could relieve acute pancreatitis in rats by inhibiting the production of TNF- α and IL-1 β. Key words: Xuebijing; pancreatitis; tumor necrosis factor- α; interleukin-1 β 肿瘤坏死因子 α(TNF- α) 、干扰素 1β(IL-1 β) 是引起组织细胞损伤的重要细胞因子，两者均参与急性胰腺炎的病理过程。近年来已有关于血必净对血 液中细胞因子影响的报道 [1-2] ，但血必净对急性胰腺炎时胰腺组织 TNF-α、 IL-1 β 的表达有无影响尚未见报道。本实验旨在通过观察血必净注射液对大鼠胰腺组织 TNF-α 、IL-1 β 表达的影响，探讨血必净对大鼠急性胰腺炎的保护作用及可能机制。 材料和方法 1.1 动物模型与分组 将 SD大鼠 48 只 ( 体重 200～250 g, 由徐州医学院实验动物中心提供 ) 随机分为假手术组、胰腺炎组和治疗组 (n=16) ，假手术组仅行 开关腹手术，胰腺炎组经胰胆管逆行注射 5%牛磺胆酸钠 (2 ml/kg ，美国 Sigma 公司 ) 制作胰腺炎模型，治疗组在胰腺炎模型基础