甲醛诱导MRC5细胞适应性反应蛋白质研究.docVIP

甲醛诱导MRC5细胞适应性反应蛋白质研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：甲醛诱导MRC5细胞适应性反应蛋白质研究 1 1 材料与方法 3 12 方法 3 2 结果 4 3 讨论 5 文2：丁酸钠诱导Raji细胞基因表达的变化 6 1材料和方法 7 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 甲醛诱导MRC5细胞适应性反应蛋白质研究 文1：甲醛诱导MRC5细胞适应性反应蛋白质研究 Proteomie analysis of adaptive respoe induced by low concentration of formaldehyde in human embryonic fibroblasts cells Abstract： Objective To study the adaptive respoe induced by formaldehyde(FA) on eukaryocyte and its possible After FA treatment，AlamarBlue reducing rate was observed to determine the cell total cellular protei were separated using two-dimeional gel electrophoresis and visualized by sliver from different batches of control and FA-treated MRC-5 cells were analyzed for quantitative spot compariso with the ImageMaster 2D platinum differentially expressed prorein spots were picked and digested in gel then identified by tandem mass The results of AlamarBlue reducing rate showed that low concentration of FA induced adaptive respoe on MRC-5 spots were changed significantly after FA protei were identified by peptide mass fingerprinting or peptide sequence analysis，including putative nucleoside diphosphate kinase，elongation factor 1-gamma，triosephosphate isomerase，60S acidic ribosomal protein P0，heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein，annexin I，hypothetical protein FLJ34423，microtubule-actin crosslinking factor 1，lamin B2，ATP synthase alpha chain，mitochondrial precuor，proteasome subunit alpha type identified protei involved in energy metabolism，tralation and RNA processing,protein folding，redox regulation,cell structure and cell Adaptive respoe can be induced by low concentration of FA on MRC-5 cells and cellular adaptation is a complex process involving in a modulation of divee cellular functio. Key words： formaldehyde；adaptive respoe；proteomics 适应性反应（adaptive respoe）是机体受到低剂量环境因子预刺激后对后续高剂量的刺激产生耐受的现象。研究表明〔1〕，许多环境刺激因子低剂量接触可诱导适应性反应。但适应性反应是否为 自然 界普遍存在的现象，适