胰岛素强化治疗对高血糖烫伤和创伤大鼠肝核转录因子κB的影响临床医学.docVIP

胰岛素强化治疗对高血糖烫伤和创伤大鼠肝核转录因子κB的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：胰岛素强化治疗对高血糖烫伤和创伤大鼠肝核转录因子κB的影响临床医学 1 1 材料与方法 3 2 结果 4 3 讨论 5 文2：核转录因子κB抑制因子对中性粒细胞凋亡的影响临床医学 6 1 材料与方法 7 2 结 果 9 3 讨 论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 胰岛素强化治疗对高血糖烫伤和创伤大鼠肝核转录因子κB的影响临床医学 文1：胰岛素强化治疗对高血糖烫伤和创伤大鼠肝核转录因子κB的影响临床医学 【Abstract】 Objective To investigate the effect of iulin inteive threapy on NF-κB expression in the live of hyperglycemic scald and trauma rats . Methods 60 male Wistar rats were received intraperitoneal injection of streptozotocin to establish diabetic model. Then they were randomized into 4 groups:scald inteive therapy group(n=15) and scald conventional therapy group(n=15). trauma inteive therapy group(n=15) and trauma conventional therapy group(n=15). Blood glucose was maitained at the level of ～/L in the inteive therapy group ,and 10～12mmol/L in the conventional therapy group. At the 10th day after therapy ,the live were collected, then the expression of NF-κB was observed by The expression of NF-κB in scald and trauma inteive therapy group was significantly lower than that in the conventional therapy group(P).Conclusion The expression of NF-κB in scald and trauma inteive therapy group was significantly lower than that in the conventional therapy group, which suggest that iulin inteive therapy can inhibit inflammatory respoe better than conventional therapy, and exert protection to the body. 【Key words】 hyperglycemic rats;iulin inteive therapy; scald and trauma; nuclear tracription factor-κB; liver 近年的研究已证明，糖尿病患者发生危重症的频率和需要手术干预的机会都比非糖尿病患者要多［1］，而国外在糖尿病危重症的抢救中［1，2］，胰岛素强化治疗较常规治疗可提高其抢救成功率和降低死亡率的优势已见报道。NF-κB是近年来发现的核转录因子，其转录调节的连接点位于许多促炎细胞因子与免疫调节的启动区，对诱导重症疾病的炎症反应起着重要作用。国内罗氏等［3］发现，创伤可明显增强脾细胞NF-κB的DNA结合活性和p65蛋白亚型的表达，在创伤后淋巴细胞活化及全身炎症反应综合征（SI）的发生、 发展 过程中可能起重要作用。为探讨在糖尿病危重症中胰岛素强化治疗对肝脏NF-κB的影响特做以下实验研究。 1 材料与方法 模型制备 Wistar雄性大鼠60只，购自山东大学实验动物中心，鼠龄8～12周，体重220～240g，清洁级饲养，大鼠禁食12h后按55mg/kg体重腹腔注射链脲佐菌素（购自Sigma公司），3天后测尾静脉血糖/L为糖尿病成功模型，再把糖尿病大鼠制成烫伤或创伤模型。烫伤模型