agp手性柱应用实例.pptVIP

AGP手性柱应用实例;Enantioselectivity can be induced and improved by simple changes of the mobile phase composition. ;Optimization of enantioselectivity and retention;Most important tool in method development : ;Net charge of AGP at different pH;Fig. 5;Retention;pH effects - propranolol;;;;Influence of uncharged modifier concentration on retention and enantioselectivity;Effect of organic modifier character on enantioselectivity;Influence of the type of organic modifier on the enantioselective retention of clevidipine;;Influence of the buffer conc. on resolution and retention of the enantiomers of naproxen;Influence of acetate concentration on retention and enantioselectivity of propranolol Column: CHIRAL-AGP 100x4.0 mm Mobile phase: 0.5 % 2-propanol in acetate buffer pH 4.1 Acetate mM k′1 k′2 a 12 5.73 7.29 1.27 25 6.54 8.79 1.34 96 7.04 10.7 1.52 ;LC/MS The type and concentration of buffer is important when developing methods for MS-detection Methods based on phosphate buffers or other nonvolatile buffers can easily be transformed to MS compatible methods by changing to ammonium acetate or ammonium formate buffers. ;Fast chiral separation suitable for MS detection;Rapid separation of acidic compounds using MS-compatible conditions;Charged organic modifiers can be an important tool in method development. They have the most dramatic effects on the enantioselectivity and the retention. Examples of modifiers: Cationic: N,N-dimethyloctylamine (DMOA) and other amines Anionic: Hexanoic- and octanoic acid;Influence of DMOA concentration on the enantioselectivity of naproxen;;Influence of the concentration of an anionic modifier(octanoic acid) on the enantioselectivity of atropine;Simple method development strategy;;Compound type;;;Roxifiban;;;;;;;;;Conclusions;If you are interested in more information please visit our website: Hundreds of Applications(all conditions given) Hundreds of literature references For a FREE Chiral S