去细胞肌肉组织工程支架与人羊膜上皮细胞的相容性研究.docVIP

去细胞肌肉组织工程支架与人羊膜上皮细胞的相容性研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：去细胞肌肉组织工程支架与人羊膜上皮细胞的相容性研究 1 1 材料与方法 3 2 结 果 5 3 讨 论 5 文2：职业技能大赛与人才培养研究 7 一、三机制 7 二、“三优化”—技能大赛促进专业人才培养与专业建设水平 7 三、“三提升”—专业人才培养模式改革助推技能大赛训练成效 8 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 11 正文 去细胞肌肉组织工程支架与人羊膜上皮细胞的相容性研究 文1：去细胞肌肉组织工程支架与人羊膜上皮细胞的相容性研究 【Abstract】 Objective To produce the acellular muscle as biomaterial scaffold and to observe its biocompatibility with the human amniotic epithelial cell.　Methods The acellular muscle scaffolds were made by extraction with 3% Triton X100 and 1% sodium dodecyl sulfate. The histological structure of acellular muscle was observed in frozen section. Then the cultured human amniotic cells were implanted into acellular muscle scaffolds. After cultured 1 w, the scaffolds with cells were sectioned, and brdU，NT3 and BDNF immunohistochemical staining were performed to observe the proliferating capacity and the expressio of NT3 and BDNF of amniotic cells in scaffold. SEM was performed to examine the distribution of human amniotic epithelial cells growing in scaffold.　Results The cells in scaffold completely disappeared after the muscle had been extracted. The scaffold of acellular muscle was arranged in parallel tubules composed of collagen fibe and elastic fibe which were left intact. In scaffold the human amnion cells could proliferate and synthesize NT3 and BDNF. Scanning electron microscopy demotrated a uniform distribution of cells in scaffold.　Conclusio The cells in rat skeletal muscle are successfully removed by chemical extraction. The acellular muscle scaffold made in this way possess good compatibility with human amniotic cells. 【Key words】 Acellular muscle; Human amniotic cell; Biocompatibility 近年来组织工程研究的重要进展之一就是采用自体或异体移植物制作天然生物降解材料的组织工程支架。其中去细胞移植物与机体有良好的生物相容性。去细胞肌肉支架可作为生物工程支架支持神经细胞轴突再生。Mligiliche等〔1〕把去细胞肌肉移植入大鼠坐骨神经缺损处,4 w后发现有大量神经轴突长入去细胞肌肉支架中。由于单独应用去细胞肌肉支架 治疗 神经系统疾病的效果有限，去细胞肌肉支架要发挥更大的作用往往需要向支架中植入种子细胞〔2，3〕。研究表明羊膜上皮细胞可分泌多种神经因子〔4，5〕，促进神经