牛耳枫与辣蓼提取物药效学研究.docVIP

牛耳枫与辣蓼提取物药效学研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：牛耳枫与辣蓼提取物药效学研究 1 1 材料与仪器 2 2 方法及结果［1，2］ 3 3 讨论 6 文2：疱疹灵药效学研究 7 1 实验材料 8 2 方法与结果 8 3 结论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 牛耳枫与辣蓼提取物药效学研究 文1：牛耳枫与辣蓼提取物药效学研究 Abstract：ObjectiveTo study the anti-acute gastroenteritis effect of Nuerfeng Lialiao Water extract (NLWE) of mouse .MethodsThe anti-inflammatory and analgesic effect of NLWE was observed by the permeability of abdominal capillary by injecting glacial acetic acid and hot plate respectively. Effect of NLWE on the contraction of smooth muscles induced by acetylcholine (Ach) or barium chloride (BaCl2) was investigated in ileum in vitro. Protective effect of NLWE was observed on absolute ethanol-induced gastric mucosa damage. ResultsNLWE had the action of reducing the permeability of abdominal capillary by injecting glacial acetic acid （P）, and had no analgesia effect . NLWE could markedly restrain the contraction of ileum smooth muscles caused by BaCl2（P ）, but had little influence on that caused by Ach. NLWE could significantly inhibit the formation of acute gastric ulcer induced by absolute ethanol in has significant anti-cnflammatory can nhibits contraction of smooth muscles and protects stomach of mouse from injury induced by ethanol. Key words：Nuerfeng Lialiao Water extract; Anti-inflammation; Analgesia; Spasmolysis 枫蓼肠胃康颗粒剂由牛耳枫Daphniphullum calucinum Benth和辣蓼Polygonnm Hydropiper Linn两味中药组成，临床用其 治疗 急性胃肠炎、食滞胃痛等症，具有良好效果，但该产品药效物质尚不明确，不能很好地阐明其作用机理。本实验针对此问题按照原处方对牛耳枫和辣蓼的提取物上大孔吸附树脂后的水洗脱部分（Nuerfeng Laliao Water Extract 简称 NLWE）进行药效学研究，进一步明确肠胃康颗粒剂的药效部位，为新药研发提供依据。 1 材料与仪器 试药肠胃康颗粒剂（海口市制药厂生产，批号060709，规格：每袋袋装8 g）；牛耳枫与辣蓼提取物(NLWE)（黑色粉末，自制，每克样品相当于原生药 g ）；阿司匹林肠溶片（昆明云健制药有限公司生产，批号，规格25 mg）；硫酸阿托品注射液（天津药业集团新郑股份有限公司生产，批号，规格：2 ml∶1 mg）；伊文思蓝（ 中国 医药集团上海试剂公司生产，批号，规格 g）；乙酰胆碱（Ach）（上海三爱思试剂有限公司生产，批号，规格：1）；氯化钡（BaCl2）（广州化学试剂厂生产，批号040503-2，规格500 g） 动物昆明种小鼠，体重18~22 g，合格证号2006A070；Wistar大鼠，体重180~220 g，合格证号2006A07；豚鼠，体重250~300 g，合格证号2006A067。以上动物均由中山大学