糖基化终末产物对人表皮角质形成细胞周期调控的影响机制.docVIP

糖基化终末产物对人表皮角质形成细胞周期调控的影响机制 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：糖基化终末产物对人表皮角质形成细胞周期调控的影响机制 1 1 AGEs的制备 3 2 细胞培养 3 3 MTT检测细胞增殖活性 4 4 流式细胞仪检测细胞周期和凋亡 4 5 siRNA干扰 4 6 细胞核酸提取及PCR检测 5 7 统计学处理 6 1 细胞增殖活性 6 2 细胞凋亡 7 3 细胞增殖周期 7 4 siRNA干扰 7 文2：姜黄素对人食管癌Ec9706细胞凋亡的影响机制医学 11 1材料和方法 11 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 17 正文 糖基化终末产物对人表皮角质形成细胞周期调控的影响机制 文1：糖基化终末产物对人表皮角质形成细胞周期调控的影响机制 Abstract： Objective To explore the mechanisms of disordered cell cycle in keratinocytes that were mediated by advanced glycation end pruducts (AGEs) in Human epidermal keratinocytes(HEK) were incubated with or without AGEs(150mg/ml),and 150mg/ml BSA(bovine serum albumin) was supported in the normal medium as controls in this 48 hou of incubation,cell apoptosis and cell cycle were detected through flow cytometer,and the cell growth activity was assayed by MTT recepto of AGEs(RAGE) were interrupted by siRNA,and the silencing efficiency was affirmed with realtime expressio of RAF1,FAK,cyclinD1,cyclinB1,CDK4 on mRNA levels were examined with RTPCR before and after gene The reduced cell proliferation and increased apoptosis were found by AGEs (150mg/ml) percentages of G2 stages in cell cycle were augmented significantly and the expressio of cell cycle relative facto,such as RAF1,cyclinD1,cyclinB1,CDK4,were raised significantly on mRNA levels mediated by AGEs before genes there were no statistical differences in these facto between AGEs group and control ones after RAGE were interrupted by In this study model,the phenomena of increased cell cycle relative facto and inhibited cell proliferation might be one of the endogenous compeating mechanisms in keratinocytes treated with mechanism was correlated with RAGE signal may have impact on cell cycle in other pathways except above relative facto in the study. Key words:AGEs;cyclinD1;cyclinB1;CDK4;cell proliferation 随着人类生活模式的改变，糖尿病已成为新世纪的慢性流行病，目前全世界糖尿病人已近2亿，预测到2025年将上升到亿[1]。随着糖尿病慢性病程的延长，其并发症发生也越来越