诱生型一氧化氮合酶和内皮素1在内毒素休克家兔血管反应性变化中的作用临床医学.docVIP

诱生型一氧化氮合酶和内皮素1在内毒素休克家兔血管反应性变化中的作用临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：诱生型一氧化氮合酶和内皮素1在内毒素休克家兔血管反应性变化中的作用临床医学 1 1 主要试剂及仪器 3 2 动物模型及分组 3 3 离体血管反应性测定 4 4 RTPCR检测iNOS和ET1mRNA水平 5 5 统计学处理方法 5 1 内毒素休克家兔SMA、CA和LRA血管反应性变化 5 文2：子宫腺肌病患者诱生型一氧化氮合酶表达的研究 9 1资料与方法 10 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 诱生型一氧化氮合酶和内皮素1在内毒素休克家兔血管反应性变化中的作用临床医学 文1：诱生型一氧化氮合酶和内皮素1在内毒素休克家兔血管反应性变化中的作用临床医学 Abstract： Objective To explore the roles of inducible nitricoxide synthase (iNOS) and endothelin1 (ET1) in the changes of vascular reactivity following endotoxic shock in The 128 rabbits were divided into 3 groups：lipopolysaccharide(LPS) control group(n=48),LPS+aminoguanidine(AG) group(n=40),LPS+PD142893 group(n=40).LPS control group was further divided into 6 subgroups: normal control,,1,2,4,6 hou after LPS superior mesenteric artery (SMA),celiac artery (CA) and left renal artery(LRA) from each animal were adopted to assay the vascular contractile and relaxing reactivity and the mRNA expression of iNOS and ET+AG group and LPS+PD142893 group both were further divided into 5 subgroups: normal control,1,2,4,6 hou after LPS vascular reactivity of SMA,CA and LRA was The increase of contractile reactivity and relaxing reactivity of CA was more than that of SMA and LRA in the early stage of endotoxic shock,and the expression level of iNOS mRNA of CA was higher than that of SMA and LRA,while the expression of ET1 mRNA of the three arteries had no significant loss of contractile reactivity and relaxing reactivity of SMA and LRA was more than that of CA in the late stage of endotoxic shock,and the expression level of iNOS and ET1 mRNA of SMA and LRA was higher than that of reactivity was significantly improved by inhibiting iNOS and ET1 The organ differential expression of iNOS and ET1 may contribute to the organ diveity of vascular hyporeactivity. Key words:endotoxic shock;vascular reactivity;iNOS;endothelin1;aminoguanidine 内毒素休克是严重创伤失血及其它临床