大鼠肝脏撞击伤动物模型的制备及评价.docVIP

大鼠肝脏撞击伤动物模型的制备及评价 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大鼠肝脏撞击伤动物模型的制备及评价 1 1 实验试剂、动物、仪器 3 2 模型制备 3 3 分组 4 4 观察指标 4 5 统计学方法 5 1 大鼠致伤前后生命体征变化 5 2 血清中ALT、AST含量 5 3 腹腔内出血量 6 4 脏器伤情特点及肝脏损伤分级 6 5 大鼠致伤后存活情况 6 文2：脑缺血再灌注动物模型的制备及评价 8 1 材料与方法 9 2 结果 12 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 大鼠肝脏撞击伤动物模型的制备及评价 文1：大鼠肝脏撞击伤动物模型的制备及评价 Abstract: Objective To establish and evaluate a new type model of blunt impact injury of liver in Totally 42 healthy SpragueDawley rats were randomly divided into 3 groups(mild injury group,moderate injury group and severe injury group).By the improved biological impact machine with a Ttype secondhit head,3 groups of hepatic bluntimpact injury model were established respectively with three differentweight steel each group was randomly divided into A group and B group on account of the difference of items difference in vital sig before and after injury and the number of natural survival animals after injury in A group were ,ALT content in serum before and after injury and the amount of intraabdominal hemorrhage of animals after injury in B group were injury of all rats was classified and given a score after the The average femoral artery pressure after injury in each group was significantly lower than that before injury(P),and decreased as the degree of injury increased(P or P).The serum levels of AST,ALT concentration after injury in each group were significantly higher than those before injury(P or P),and increased with degree of injury(P or P).The amount of intraabdominal hemorrhage in each group significantly increased with degree of injury(P or P).The score for the hepatic injury significantly increased with degree of injury(P).Conclusion The degree of liver damage in each group obviously increases with steel ball rat liver trauma model is an ideal experimental animal model. Key words:liver injury;impact injury;animal model 撞击伤一般是指生物机体与其他物体相互撞击而引起的机体的钝性损伤。它是平时意外事故(如高空坠落、