实时定量逆转录聚合酶链反应检测大肠癌患者术前外周血CK20mRNA和CEAmRNA的意义临床医学.docVIP

实时定量逆转录聚合酶链反应检测大肠癌患者术前外周血CK20mRNA和CEAmRNA的意义临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：实时定量逆转录聚合酶链反应检测大肠癌患者术前外周血CK20mRNA和CEAmRNA的意义临床医学 2 1 对象与方法 3 2 结果 4 3 讨论 5 文2：实时定量逆转录聚合酶链反应检测大肠癌患者术前外周血CK20mRNA和CEAmRNA的临床意义 7 1 对象与方法 8 2 结果 9 3 讨论 10 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 实时定量逆转录聚合酶链反应检测大肠癌患者术前外周血CK20mRNA和CEAmRNA的意义临床医学 文1：实时定量逆转录聚合酶链反应检测大肠癌患者术前外周血CK20mRNA和CEAmRNA的意义临床医学 ［Abstract］ Objective To investigate the expression of CK20 mRNA and CEA mRNA in peripheral blood of patients with colorectal cancer and its clinical CK20 mRNA and CEA mRNA were detected in 68 patients with colorectal cancer before operation,30 health adults by CK20 mRNA and CEA mRNA were undetected in the control group,the positive rate of CK20 mRNA among colorectal cancer patients was %,and of CEA mRNA was %.The positive rate of combination detection was %.It was higher than the rate of detection was no statistically significant difference of positive rate between two detection expression of CK20 mRNA and CEA mRNA were not significantly correlated with sex of patients,the place of the tumor or differentiation level of cancer cells,but correlated with clinical stage,lymph nodes metastasis and liver CK20 mRNA and CEA mRNA were useful maker of blood dissemination of colorectal cancer cell and the positive rate of micrometastasis be maybe raised by combination expression of CK20 mRNA and CEA mRNA may be regarded as a reference indicator. ［Key words］ colorectal cancer;micrometastasis;peripheral blood 大肠癌是我国发病率较高的恶性肿瘤之一，其发病率呈逐年升高趋势，其术后复发及远处转移率达20%～45%［1］。因此早期检测出循环癌细胞对判断肿瘤复发和转移及指导临床综合 治疗 有重要意义［2］ 1 对象与方法 研究对象 68例大肠癌患者选自2007年1月～2008年3月在我院接受手术治疗并经病理确诊的患者，男39例，女29例，年龄26～88岁，中位年龄65岁。临床分期：Ⅰ期6例，Ⅱ期24例，Ⅲ期27例，Ⅳ期11例。肿瘤分化程度：高分化腺癌26例，中分化28例，低分化11例，未分化3例。对照组30例健康志愿者来自同期我院健康职工。 仪器与试剂 定量PCR仪（美国生物应用公司ABI7500），Trizol试剂（上海闪晶分子生物科技公司），CK20 mRNA和CEA mRNA PCR试剂盒（上海达为科生物科技公司） RNA提取 采集对照组及大肠癌组患者术前肘静脉血5 ml（草酸钾抗凝），1 h内用淋巴细胞分离液分离有核细胞，将分离的有核细胞收集到 ml离心管中，12 000 rpm离心5 min,弃上清，在沉淀中加入T