骨髓CD34加干细胞种植人工血管应用于血管置换后血清中6kPGF1α和TXB2变化的研究临床医学.docVIP

骨髓CD34加干细胞种植人工血管应用于血管置换后血清中6kPGF1α和TXB2变化的研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：骨髓CD34加干细胞种植人工血管应用于血管置换后血清中6kPGF1α和TXB2变化的研究临床医学 2 1 对象与方法 3 2 结果 3 3 讨论 4 文2：骨髓CD34加干细胞种植人工血管应用于血管置换后内皮化和通畅率的研究临床医学 7 1　材料与方法 8 2　结果 9 3　讨论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 14 正文 骨髓CD34加干细胞种植人工血管应用于血管置换后血清中6kPGF1α和TXB2变化的研究临床医学 文1：骨髓CD34加干细胞种植人工血管应用于血管置换后血清中6kPGF1α和TXB2变化的研究临床医学 [Abstract] Objective To investigate the relatiohip between the 6-Keto-PG F1α，TXB2 and the endotheliosis and thrombosis of the new endomembrane on the prostheses seeded by CD34+ stem Twelve dogs were randomized into two covered with ePTFE or Dacron were implanted into the abdominal aorta(AA) and inferior vena cava (IVC).In each group，6 dogs were implanted by prostheses seeded by CD34+ stem cells，4 dogs were implanted by autogenous blood only as blood was collected in preoperative and 3，7，14，30，60 days postoperative from femoral and electron microscopy were applied to observe the endotheliosis and thrombosis of the new endomembrane when harvesting all the prostheses in the 60 The levels of the platelet，6-Keto-PGF1α，TXB2 and P/T in experimental group was significantly different than the control group. The thickness of new endomembrane in control group was notablely different than the experimental It can restrain the lower levels of 6-Keto-PGF1α and P/T and the higher concentration of TXB2 platelet in experimental group and then inhibit endotheliosis and thrombosis in the prostheses covered with ePTFE or Dacron that are implanted by CD34+ stem cells than the controls. [Key words] prostheses;CD34;bona marrow;prostacyclin;thromboxane B2 血管旁路术后血管壁内膜的损伤，能引起血流动力学改变、血小板的激活和细胞因子的释放，造成内膜的反应性增生，常可引起术后中晚期的狭窄或闭塞[1]。骨髓CD34+干细胞种植人工血管应用于血管置换，可有效并尽早形成内膜，防止术后内膜过度增生致狭窄或闭塞。本文检测血清中6-k-PGF1α 和TXB2的变化情况更进一步说明了骨髓CD34+干细胞种植人工血管应用于血管置换可能有防止内膜增生和血栓形成的作用。 1 对象与方法 血标本采集及观察指标 选杂种犬12条，依人工血管不同分为ePTFE血管实验组(4条)和涤纶血管实验组(4条)及ePTFE对照组(2条)和涤纶对照组(2条)，每只犬在术前和术后3、7、14、30、60天股静脉采血分别放入按要求加入相应抗凝剂的3个不同试管中。应