西维来司钠对高原颅脑损伤大鼠中性粒细胞弹性蛋白酶的影响及神经损伤的保护作用临床医学.docVIP

西维来司钠对高原颅脑损伤大鼠中性粒细胞弹性蛋白酶的影响及神经损伤的保护作用临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：西维来司钠对高原颅脑损伤大鼠中性粒细胞弹性蛋白酶的影响及神经损伤的保护作用临床医学 2 1 动物及分组 3 2 平原大鼠颅脑创伤模型制备 3 3 高原大鼠颅脑创伤模型制备 3 4 颅脑创伤后治疗 4 5 NE浓度测定 4 6 病理学检查 4 7 统计学处理 4 2 高原颅脑创伤大鼠给予西维来司钠治疗前后脑组织形态学变化 5 1 高原颅脑损伤重于平原与NE相关 5 2 NE抑制剂西维来司钠的神经保护作用 7 文2：西维来司钠对大鼠脑缺血再灌注损伤的保护作用临床医学 8 1 材料与方法 9 2 结果 11 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 西维来司钠对高原颅脑损伤大鼠中性粒细胞弹性蛋白酶的影响及神经损伤的保护作用临床医学 文1：西维来司钠对高原颅脑损伤大鼠中性粒细胞弹性蛋白酶的影响及神经损伤的保护作用临床医学 Abstract： Objective To investigate the effects of neutrophil elastase(NE) in traumatic brain injury and neuroprotective effect of sivelestat in rats at simulated high Totally 48 adult male Wistar rats were divided into plain group and plateau group,and then subdivided into control group,craniocerebral injury group and craniocerebral injury+sivelestat therapy group in both groups model of traumatic brain injury at both simulated high altitude and plain was made by Feeney’s level of NE before and after the craniocerebral injury between plateau group and plain group was observed and the diveity of NE between the two groups after the treatment was analyzed by injecting neuroprotective effect of sivelestat at the simulated high altitude group was observed in The levels of NE before and after injury in plateau groups were obviously higher than those in the plain groups(P).HE dying of the brain showed that sivelestat had the neuroprotective effects in craniocerebral brain injury at simulated high NE is an important factor in traumatic craniocerebral injury at simulated high altitude,and sivelestat has an obvious neuroprotective effect in traumatic craniocerebral injury at simulated high altitude. Key words:craniocerebral injury;elastase;sivelestat;nerve injury;plateau 颅脑损伤是高原地区常见的神经外科急症，由于受高原严寒低氧、低气压等恶劣环境影响，患者病情危重，病程进展快，以致高原颅脑损伤致残率及病死率均明显高于平原地区。研究表明，中性粒细胞弹性蛋白酶(netrophil elastase,NE)在颅脑损伤后表达水平增高，且与损伤程度成正比，并加重颅脑损伤[1]。为此本研究拟探讨NE是否为高原颅脑损伤病情迅速恶化的重要因素，以及给予NE