淀粉样前体蛋白酶与β淀粉样蛋白在衰老痴呆大鼠脑组织中的表达临床医学.docVIP

淀粉样前体蛋白酶与β淀粉样蛋白在衰老痴呆大鼠脑组织中的表达临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：淀粉样前体蛋白酶与β淀粉样蛋白在衰老痴呆大鼠脑组织中的表达临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：胰岛素样生长因子与β淀粉样蛋白在糖尿病大鼠脑组织中的表达及意义临床医学 6 1 材料与方法 7 2 结 果 9 3 讨 论 10 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 淀粉样前体蛋白酶与β淀粉样蛋白在衰老痴呆大鼠脑组织中的表达临床医学 文1：淀粉样前体蛋白酶与β淀粉样蛋白在衰老痴呆大鼠脑组织中的表达临床医学 【Abstract】 Objective To investigate expression of βamyloid precuor protein (APP) and βamyloid protein (Aβ) in brain of rat with aged dementia and study the mechanism of APP and Aβ in aged Wistar rats were randomly divided into normal group，shamoperation group，4，6 and 8 weeks ageddementia model and cognitive changes of rats were tested by Morris water maze and shuttle box of AβPP was measured by ELISA and Western blotting，and the level of Aβ in brain tissue was detected by light deity value was measured by imaging The expressio of APP and Aβ in model groups were higher than those of normal and shamoperation groups(P＜)，and there were no significant differenc among three model groups in the expressio of APP and Aβ(P＞).The expression of APP and Aβ had no correlation in level of APP had oppositive correlation with learning and remembering and the level of Aβ had negtive correlation with learning and There are high expressio of APP and Aβ in aged dementia rats.，which suggest APP play a neuroprotection role in aging process and Aβ participate in the pathogenesis of aged dementia，but no pathological significance. 【Key words】 Aged dementia；βamyloid precuor protein；βamyloid protein 阿尔茨海默病(AD)的病理特征是以β淀粉样蛋白(Aβ)沉积为核心形成的老年斑，脑内Aβ是由淀粉样前体蛋白(APP)经一系列分泌酶降解而来，所以对于APP、Aβ在AD中的发病机制研究尤为深入，而AD为年龄衰老相关性疾病〔1～3〕，那么作为Aβ沉积为核心的AD病理改变在老化机制有何联系，APP与衰老老化有什么联系国内外未见报道。因此，我们这里选择自由基中毒老化鼠作为我们研究对象，检测痴呆衰老大鼠脑内海马组织APP、Aβ的表达，初步探讨APP、Aβ的表达和痴呆衰老的联系。 1 材料与方法 动物 Wistar成年大鼠30只，雌性，体重200～250 mg，第三军医大学野战 医院 动物研究所提供，动物中心许可证号：渝实动设施准第260号。 方法 试剂与仪器 APP、Aβ40/42：Biosource International，Inc.，USA；D半乳糖(Dgal)：上海试剂二厂；大鼠跳台和大鼠穿梭箱程序自动控制仪均由重庆市神经病学重点实验室和重庆医科大学药学院提供。 模型制