龙眼核醋酸乙酯部位的抗氧化活性研究.docVIP

龙眼核醋酸乙酯部位的抗氧化活性研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：龙眼核醋酸乙酯部位的抗氧化活性研究 1 1 材料与仪器 2 2 方法 3 3 结果 5 4 讨论 7 文2：金花茶化学成分的抗氧化活性研究 7 1金花茶化学成分及作用 8 2金花茶——天然抗氧化剂 8 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 龙眼核醋酸乙酯部位的抗氧化活性研究 文1：龙眼核醋酸乙酯部位的抗氧化活性研究 Abstract：ObjectiveTo investigate the anti-oxidative effect of chemical cotituents in Longan seed，ethyl acetate fraction. MethodsDifferent fraction cotituents of longan seed were obtained by isolating with ethanol solvent, extracting with ethyl acetate and purifying with colomn chromatography and recrystallization and so on. With the help of TBA spectrometric method, effects of ethyl acetate extract from Longan seed on lipid peroxidation were measured by the determination of malondialdehyde (MDA) that was produced by the lipid peroxidation of liver tissue homogenate and mitochondria induced by cysteine-Fe2+system and on scavenging of hydroxyl radical were determined by Fenton system. ResultsSixteen cotituents of longan seed , ethyl acetate fraction(Fr1～Fr16) were obtained. Fraction Fr8～Fr15 could inhibit the lipid peroxidation of liver tissue homogenate and the lipid peroxidation of mitochondria induced by Fe2+-Cys and scavenge hydroxyl radical. Some fractios activities are in dose-dependent manne. Conclusion Cotituents of longan seed , ethyl acetate fraction have good antilipid-peroxidation effect and scavenge hydroxyl radical activity. Key words：Longan seed; Anti-oxidation effect; Free radicals 龙眼Dimocarpus longan lour.俗名桂圆，是无患子科龙眼属植物。其核具有止血、定痛、理气化湿，治创伤出血、疝气等［1］和治狐臭作用［2］，近期有研究表明龙眼核的提取物有降血糖的作用［3］，龙眼果肉及龙眼多糖具有抗氧化的作用［4，5］，但龙眼核中化学成分的抗氧化作用研究尚未见报道。为了对龙眼核作进一步的开发利用，本实验对龙眼核进行了体外抗氧化作用的研究。现报道如下。 1 材料与仪器 材料与试剂 实验用的原料为石硖龙眼干的核，经广西中医药研究所严克俭助理研究员鉴定，为无患子科植物龙眼的干燥果实。实验用测试样品为龙眼核醋酸乙酯浸膏，再经硅胶柱层析洗脱而得的各流份。 SD大鼠，体重200～300 g，由广西医科大学实验动物中心提供。 硫代巴比妥酸（TBA）、盐酸、考马斯亮蓝、硫酸亚铁、半胱氨酸、磷酸水杨酸、过氧化氢等试剂均为国产分析纯试剂。 仪器 设备752N紫外可见分光光度计、FJ-200高速分散匀质机（上海标本模型厂制造）、3145台式高速冷冻离心机、数显式三用电热恒温水温箱（上海跃进医疗机械厂）、精密 电子 天平、旋转蒸发仪（一台为RE-52AA，上海亚荣生化仪器厂生产；另一台为上海医械专机厂生产