TGFβ1基因转染BMSCs后复合软骨细胞共培养临床医学.docVIP

TGFβ1基因转染BMSCs后复合软骨细胞共培养临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：TGFβ1基因转染BMSCs后复合软骨细胞共培养临床医学 1 1 材料与方法 3 2 结果 6 3 讨论 7 文2：TGFβ1基因转染可抑制人晶状体上皮细胞增殖 8 1材料和方法 9 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 TGFβ1基因转染BMSCs后复合软骨细胞共培养临床医学 文1：TGFβ1基因转染BMSCs后复合软骨细胞共培养临床医学 [Abstract] Objective To investigate the secretion of target gene and differentiation of BMSCs trafected by TGF-β1 and co-culture and together into chondrocytes and to provide a new method for culturing seed cells in cartilage tissue The plasmids pcDNA β1 were amplified and extracted，then cut by enzymes，electrophoresed and analyzed its of Wistar rats were separated and purificated by the deity gradient centrifugation and adherent morphologic changes of primary and passaged cells were observed by inverted phase contrast microscope and cell surface marke were detected by immunofluorescence to the trafect situation，the BMSCs were divided into 5 groups，the trafected group(Group A)，the co-culture group(Group B)，the group of TGF-β1 and co-culture(Group C)，the group of BMSCs(Group D)，the group of chondrocytes(Group E).After being trafected，the cells were selected，then the proliferation activity was tested by MTT and expression levels were tested by RT-PCR and Western The Group C showed that it had a good prospect and important significance of clinic application in cartilage tissue β1 gene bands，gene sequencing were coistent with Gene-Bank cDNA absorbance value with MTT determination of A，B，C，D，E group of cells in 490 nm wavelength in C group was statistically different with A，B，D，E groups(P).RT-PCR and Western blot detection were done for gene and protein expression，TGF-β1 expression in group C were most，A group followed by;Ⅱ collagen expression in C group was most，A group followed By TGF-β1 trafecting cells and culturing chondrocytes at the same time is effective in promoting differentiation of BMSCs to has more great significance in clinical application of tissue-engineered cartil