硫化氢对大鼠舱室内肢体爆炸伤后肺损伤的作用.docVIP

硫化氢对大鼠舱室内肢体爆炸伤后肺损伤的作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：硫化氢对大鼠舱室内肢体爆炸伤后肺损伤的作用 2 1 大鼠舱室内肢体爆炸伤模型的制作 3 2 实验分组 4 3 观察指标 4 4 统计学处理 6 1 爆炸参数和大鼠肢体爆炸伤后大体观察 6 2 大鼠肺脏TNFα、IL6、IL10浓度变化 6 4 大鼠肺含水量变化 6 5 大鼠肺组织病 理学 变化 7 6 大鼠CSE活性和H2S浓度变化 7 文2：高寒环境犬肢体爆炸伤后病理变化和感染特征研究 9 1 实验动物 10 2 实验模型及致伤条件 10 3 伤道区标本采集、细菌培养鉴定、伤道局部细菌定量检测 10 4 统计学处理 11 1 肉眼观察 11 2 镜下观察 11 3 伤后伤口细菌定量测定，细菌定性鉴定(表1、2) 12 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 硫化氢对大鼠舱室内肢体爆炸伤后肺损伤的作用 文1：硫化氢对大鼠舱室内肢体爆炸伤后肺损伤的作用 Abstract: Objective To study the effects of hydrogen sulfide(H2S) on lung damage after limb’s explosive injury in closed space in rats,and discuss the clinical value of H2S in the therapy for explosive Limb’s explosive injury in closed space was male SpragueDawley rats (200～220g) were randomly divided into simple explosive injury group (E group,n=32),explosive injury + NaHS group (S group: 5mg/kg NaHS,ip,n=16) and control group(C group:n=8).Lung and blood samples were pressure of blast injury,CSE activity in lung homogenate，H2S concentration in plasma,TNFα,IL6,IL10 concentratio in lung homogenate and lung water content were change of lung was After explosion,CSE activity in lung homogenate and H2S concentration in plasma decreased markedly(P,vs C group);TNFα,IL6,IL10 concentratio in lung homogenate and lung water content increased markedly(P or P,vs C group),and showed severe pneumorrhagia,pneumonedema,inflammatory cell infiltration and alveoli pulmonum in S group,TNFα,IL6,IL10 concentratio and lung water content decreased significantly(P or P,vs E group),and pneumorrhagia,pneumonedema and inflammatory cell infiltration relieved Limb’s explosive injury in closed space could lead to severe lung injecting H2S in the early stage can relieve lung damage after explosive injury. Key words:explosive injury;lung damage;limbs;hydrogen sulfide(H2S) 硫化氢(hydrogen sulfide,H2S)是一种有臭鸡蛋气味的有毒气体，但近年发现多种哺乳动物组织细胞都有内源性H2S合成酶系的表达并具备合成内源性H2S的能力;而内源性H2S