分子生物学：基因克隆、克隆基因的表达.ppt

Ligation EcoRI EcoRI RE digestion Vector EcoRI Vector RE digestion Recombinant vector Recombinant vector Recombinant vector EcoRI EcoRI EcoRI EcoRI EcoRI ① 单酶消化的粘性末端连接： 连接效率高; 载体需去磷酸; 双向连入载体 Vector SmaI HeaIII SmaI RE digestion RE digestion Vector Ligation Helf of smaI Recombinant vector SmaI Helf of HeaIII Recombinant vector SamI Helf of smaI Helf of HeaIII ② 平头末端连接： 连接效率低 双向连入载体 自身环化率高 Target gene EcoRI BamHI EcoRI BamHI Vector Vector digestion digestion Ligation