体外培养人脐带血间充质干细胞向胰岛细胞分化.docVIP

体外培养人脐带血间充质干细胞向胰岛细胞分化 文档信息 主题： 关于“医学心理学”中“妇产科学”的参考范文。 属性： Doc-02ANCP，doc格式，正文9418字。质优实惠，欢迎下载！ 适用： 作为文章写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：体外培养人脐带血间充质干细胞向胰岛细胞分化 1 1试剂与方法 3 1. 3体外诱导后胰岛细胞检测 4 1. 4胰岛样细胞移植造模糖尿病大鼠 4 2. 2诱导胰岛细胞的检测 5 文2：体外诱导人脐带血间充质干细胞为软骨细胞的初步研究 8 1 材料和方法 9 2 结果 11 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 体外培养人脐带血间充质干细胞向胰岛细胞分化 文1：体外培养人脐带血间充质干细胞向胰岛细胞分化 Inducing human umbilical cord blood mesenchymal stem cells into iulin secreting cells in vitro culture and its effects on diabetes rats WANG Ying， LUO Hao-hong， GAO Hong-quan， et al. Medical College of Xiamen， Xiamen 361008， China 【Abstract】ObjectiveTo explore the possibility of inducing the differentiation of human umbilical cord blood mesenchymal stem cells(HUCB-MSCs) into iulin secreting cells in vitro and the therapeutic efficacy on diabetic rats following traplantation. Methods?We isolated and cultured HUCB-MSCs， and induced HUCB-MSCs to differentiate into iulin secreting cells in the islet cell culture condition. HUCB-MSCs of induction were detected by immunocytochemical Methods. Zinchydronium was detected by Dithizon staining. Traplantation efficiency of islet-like cells was detected. Results?Under induction， immunocytochemical examination showed that the expression of human iulin was positive. Dithizon stain showed that the cytoplasm of HUCB-MSCs was stained in brownish red color after induction. At 2 weeks following traplantation， blood glucose concentration in the islet-like cell group significantly reduced. Conclusion?HUCB-MSCs can be differentiated into iulin secreting cells in vitro. HUCB-MSCs have the potential to become an excellent candidate in β cell replacement therapy of typeⅠdiabetes. 【Key words】Human umbilical cord blood; Mesenchymal stem cells; Induction; Diabetes rats2000年， Erices等［1］报道人脐血中含有丰富的造血干细胞和间充质干细胞(mesenchymal stem cell/ mesenchymal progenitor cell， MSC/MPC)。脐带血间充质干细胞(umbilical cord blood mesen