分子生物学第五章.ppt

In prokaryotes, transcription produces a nearly exact mRNA copy of the DNA, and the transcript is immediately translated into protein. In eukaryotes, a series of modifications occur to mRNA during and after transcription. ;5.1 Capping 5.2 Polyadenylation 5.3 Splicing 5.4 mRNA Editing 5.5 Experiments;5.1 Capping / 加帽;Structure of the cap / 帽的结构;RNA polymerase II;Capping takes place quite early.;Capping process / 加帽过程;CTD: C-terminal domain;Functions of the cap structure帽结构的功能;1. Helps prevent degradation帮助防止降解;2. Helps transport into cytoplasm帮助转运到细胞质中;3. Enhances translation