中药分析实验exp2六味地黄丸的质量检验--版本219.pptxVIP

Experiment 2 Analysis of Liuwei Dihuang WanTo comprehend the rules for the selection of components for the identification and assay of traditional Chinese patent medicines.To study HPLC method for the assay of traditional Chinese patent medicines.To study the microscopic identification method for traditional Chinese patent medicines.To study pre-treating methods for traditional Chinese medicines.IdentificationTo 9 g of small honeyed pills or big honeyed pills, or 6 g of water-honeyed pills, in powder, add 4 g of kieselguhr and triturate well. Heat under reflux with 40 ml of ether at a low temperature for 1 hour, filter and evaporate to remove ether. Dissolve the residue in 1 ml of acetone as the test solution. Dissolve paeonol CRS in acetone to produce a solution containing 1 mg per ml, as the reference solution. Carry out the method for thin layer chromatographyusing silica gel G as the coating substance and cyclohexane-ethyl acetate (3:1) as the mobile phase. Apply separately to the plate 10 ?l of each of the two solutions. After developing and removal of the plate, dry it in the air, spray with 5% solution of ferric chloride in ethanol acidified with hydrochloric acid and visualize under a current of hot air. The bluish-brown spot in the chromatogram obtained with the test solution corresponds in position and colour to that in the chromatogram obtained with the reference solution. Cortex Moutan Carry out the method for high performance liquid chromatography.Chromatographic system and system suitability Use octadecylsilane bonded silica gel as the stationary phase and a mixture of methanol and water (70:30) as the mobile phase. As detector, a spectrophotometer is set at 274 nm; the number of the theoretical plates of the column is not less than 3500, calculated with the reference to the peak of paeonol.Reference solution Dissolve a quantity of paeonol CRS, accurately weighed, in 50% methanol to prepare a solution containing 20 ?g per ml.Test solution Pulverize