抵挡汤早期干预对糖尿病大鼠腺苷酸活化蛋白激酶信号通路的影响.docVIP

抵挡汤早期干预对糖尿病大鼠腺苷酸活化蛋白激酶信号通路的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：抵挡汤早期干预对糖尿病大鼠腺苷酸活化蛋白激酶信号通路的影响 1 1 实验材料 3 2 实验方法 4 3 统计学方法 6 4 结果 6 5 讨论 8 文2：辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响 11 1 材料与方法 12 2 结果 14 24 h尿蛋白、Cr、Glu、TC、TG 14 3 讨论 15 参考文摘引言： 16 原创性声明（模板） 18 文章致谢（模板） 18 正文 抵挡汤早期干预对糖尿病大鼠腺苷酸活化蛋白激酶信号通路的影响 文1：抵挡汤早期干预对糖尿病大鼠腺苷酸活化蛋白激酶信号通路的影响 DOI：/-.017 Abstract： Objective To observe the effects of the intervention of Didang Decoction at different times on changes of AMPK signaling pathway related facto in macrovascular endotheliocytes of diabetic rats； To discuss the mechanism of mitochondria energy metabolism regulating the AMPK signaling pathway for macrovascular endothelial defee function. Methods Injection of STZ into the caudal vein and administration of high fat diet wer used to generate diabetic rat model. All rats were randomly divided into the following 7 groups： control， model， metformin， simvastatin， early-， middle-， and late-stage Didang Decoction group. Western blot was used to detect the expressio of APMKα1 and PGC-1α in rat aortic endothelial cells. Changes in the intracellular AMP and ATP levels were detected by ELISA. Real time fluorescence quantitative PCR was used to detected mRNA expressio of Caspase-3， eNOS， and Bcl-2 in tissue of thoracic aorta. Results Compared with the model group， the expressio of AMPKα1 and PGC-1α in the early-stage and middle-stage Didang Decoction group and simvastatin group increased （P）； the gene expressio of Bcl-2， and eNOS significantly increased in the early-stage Didang Decoction group and simvastatin group （P）， while the expressio of Caspase-3 decreased significantly （P）. The expression of ATP increased significantly and the expression of AMP decreased significantly in the early-stage Didang Decoction group and simvastatin group （P）， and the best effects were shown in the early-stage Didang Decoction group. Conclusion Early intervention of Didang Decoction can enhance energy metabolism i