糖基转移酶与三萜皂苷的合成研究.docVIP

糖基转移酶与三萜皂苷的合成研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：糖基转移酶与三萜皂苷的合成研究 1 1 三萜皂苷的应用前景及制备现状 4 三萜皂苷的应用前景 4 三萜皂苷的制备现状 5 三萜皂苷合成相关基因的挖掘 6 2 糖基转移酶与三萜皂苷的合成 8 3 展望 12 文2：邻苯二甲酸二异辛酯的合成研究 14 1 实验方法 15 2 结果与讨论 17 3 结论 17 参考文摘引言： 18 原创性声明（模板） 19 文章致谢（模板） 19 正文 糖基转移酶与三萜皂苷的合成研究 文1：糖基转移酶与三萜皂苷的合成研究 作者简介： 李诺楠(1995—)，女，硕士研究生，lnuonan@; 李春(1970—)，男，博士，教授，lichun@ 收稿日期：2019-05-31 基金： 国家自然科学基金重点项目( The applicatio of glycosyltraferases in the synthesis of triterpenoid saponi LI Nuonan LI Chun School of Chemistry and Chemical Engineering， Beijing Ititute of Technology The State Key Laboratory of Bioreactor Engineering， East China Univeity of Science and Technology Abstract：Triterpenoid saponi are usually composed of one or more glycosyl groups attached to hydrophobic aglycones， which are widely used as active ingredients in traditional Chinese herbal medicines. The traditional method for obtaining triterpenoid saponi is extracted from plants， due to the low content of triterpenoid saponi in plants and the limitation of plant growth by land resources， climate and environment， it has greatly restricted its large-scale promotion and application. Thus，It’s hard to be applied and popularized on large scale. The use of synthetic biology principles to design and cotruct microbial cell factories to synthesize triterpenoids is coidered to be the most promising alternative and has become a new research hotspot. Glycosyltraferases play a key role in the synthesis of triterpenoid saponi. This article will describe the research progress in the synthesis of triterpenoid saponi using glycosyltraferases， and provide a reference for the further application of triterpenoid saponi。 Keyword：triterpenoid saponi; glycosylation; glycosyltraferases; synthetic biology; Received： 2019-05-31 引 言 三萜类化合物是一类由6个异戊二烯单元（C5H8）组成的结构多样的化合物，在植物中这类化合物常以其糖基化形式存储[1]——即三萜皂苷。三萜皂苷由疏水的三萜皂苷元连接一个或多个糖基构成[2，3]（图1）。植物体内的三萜皂苷类化合物在植物体防御微生物和食草动物侵害及信号传导等方面起到重要作用[2]。现代药理学研究证实，一些三萜皂苷类化合物对人体也有很强的生理活性，因此存