抗黄曲霉毒素M1抗体制备及检测方法建立.doc

抗黄曲霉毒素M1抗体制备及检测方法建立 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：抗黄曲霉毒素M1抗体制备及检测方法建立 1 113 溶液系统 参考文献〔5〕制备。 4 12 方法 4 14 样品提取 按照国家标准方法提取〔10〕 6 2 结果 6 3 讨论 7 文2：抗黄曲霉毒素M1抗体制备及检测方法建立 8 1 材料与方法 10 11 材料 10 113 溶液系统 参考 文献 〔5〕制备。 11 12 方法 11 14 样品提取 按照国家标准方法提取〔10〕 12 2 结果 13 3 讨论 14 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 16 正文 抗黄曲霉毒素M1抗体制备及检测方法建立 文1：抗黄曲霉毒素M1抗体制备及检测方法建立 Development of monoclonal antibody agait aflatoxin M1 and immunoassay for aflatoxin M1 Abstract： Objective To prepare monoclonal antibody agait aflatoxin M1 and develop indirect competitive enzyme-linked immunosorbent assay for detection of aflatoxin Hybridoma cell line excreting monoclonal antibody agait aflatoxin M1 was produced using B cell hybridoma technique and develop indirect competitive enzyme-linked immunosorbent assay for detection of aflatoxin One hybridoma cell line excreting monoclonal antibodies agait aflatoxin M1 coded 2F2 was obtained by fusing murine Sp2/0 cells with spleen cells from BALB/c mice immunized with AFM1-BSA monoclonal antibody produced by the hybridoma cell were tested for subtype and designated as IgG1 for affinity of IgG in purified ascites yielded by hybridoma cell was 28×10-11 mol/ monoclonal antibody obtained in the present study was specific to aflatoxin M1，because of lightly cross reactio among the monoclonal antibodies agait aflatoxin M1 with the analogues of aflatoxin B1，aflatoxin B2，aflatoxin G1，aflatoxin G2 and aflatoxin M2 were limit of detecting concentration of aflatoxin M1 was ng/ linear range of standard curve was 002～2ng/ml and the linear equation was y=-04364x+02693(R2=).The recovery of aflatoxin M1 was between 725%～1313%.Conclusion The monoclonal antibody obtained in the study was of relatively high specificity to aflatoxin M1，and a simple fast seitive indirect competitive enzyme-linked immunosorbent assay for the detection of aflatoxin M1 was developed。 Key words： aflatoxin M1;monoclonal