COL1A2基因多态性与儿童氟斑牙关系.docVIP

COL1A2基因多态性与儿童氟斑牙关系 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：COL1A2基因多态性与儿童氟斑牙关系 1 1 对象与方法 3 2 结果 4 21 儿童外周血COL1A2 PvuⅡ基因多态性结果 4 22 COL1A2 PvuⅡ基因多态性与氟斑牙的关系 5 3 讨论 5 文2：COL1A2基因多态性与儿童氟斑牙关系 6 1 对象与方法 7 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 11 正文 COL1A2基因多态性与儿童氟斑牙关系 文1：COL1A2基因多态性与儿童氟斑牙关系 Relatiohip of COL1A2 polymorphism to childrens dental fluorosis Abstract： Objective To explore the distribution of collagen type 1 alpha2 (COL1A2)PvuⅡ genotype in children who lived in the high fluoride，high iodine areas，and evaluate the relatiohip of COL1A2 PvuⅡgene polymorphism to childrens dental Polymerase chain reaction-restriction fragment length polymorphism technique（PCR-RFLP） was used to determine COL1A2 PvuⅡ genotype of the different children lived in the normal fluoride and iodine area ，the high fluoride area，the high fluoride and high iodine incidence of dental fluorosis was examined by using Deans The distribution frequency of COL1A2 PvuⅡ genotype was %，%，% in the normal fluoride and iodine area，%，%，% in the high fluoride area ，and %，%，% in the high fluoride and high iodine area were no significant differences in the three areas（χ2＝，P）.The prevalence of dental fluorosis in these three groups was 0，%，% respectively，and there were significant differences in the three areas（χ2＝，），but the community fluorosis index(CFI) in the high fluoride and iodine area was lower than that in the high fluoride There were no correlation between COL1A2 PvuⅡgene polymorphism and the dental fluorosis in the three areas。 Key words： collagen type I；dental fluorosis；gene polymorphism I型胶原是骨组织中唯一的胶原，由2条α1链和1条α2链组成，分别由Ⅰ型胶原α1链(COL1A1)和Ⅰ型胶原α2链(COL1A2)基因编码。Ⅰ型胶原基因的多态性多种多样，其基因的突变可导致很多疾病。近年来对它的研究主要集中在先天遗传疾病上〔1〕。随着环境基因组计划（EGP）启动，越来越多的研究表明，骨组织的损伤反应不仅与暴露于环境有害因素的程度有关，同时还与遗传易感性或耐受性有着密切联系〔2-4〕。而有关COL1A2基因多态性的分布及其与氟斑牙发生发展关系报道较少。本次研究通过环境流行病学调查，采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法，分析Ⅰ型胶原α2链（COL1A2）PvuⅡ各基因型在地氟病区（单纯高氟区、高氟高碘区）儿童中的分布，探讨该位点基因多态性与儿童氟斑牙的关系，为地氟病防治提供基础资料。 1 对象与方法 11 对