组氨酸脱羧酶.pdfVIP

组氨酸脱羧酶(HDC)催化作用组胺的形成,生物活性胺。代理控制 HDC 活动是有益的治疗 histamine-mediated 症状,如过敏和肠胃溃疡。 我们的结果表明,食品包含强大的 HDC 抑制剂,这些食品有效成分可能是有用的设计临床可 用HDC 抑制剂。 组胺是一种生物活性胺化学介质的过敏反应 ,神经递质 ,和胃酸分泌的诱导物 (Shahid Khardori,汗,和非洲2011)。组氨酸脱羧酶(HDC。4.1.1.22)是唯一的酶参与了哺乳动物的组 胺合成。抑制体内 HDC 活动降低组胺水平;因此,抑制 HDC 的代理活动将是有用的治疗 histamine-mediated 症状,如过敏、胃肠溃疡和炎症。然而,尽管收到重视,寻找HDC 抑制剂 没有足够成功,可能是因为困难处理酶和HDC 的少量研究组织 由于组胺释放是在许 多炎症 反应 的主要活动之一 , 一个新 的潜在 的应用 (-)-epigallocatechin-3-gallate 在预防或治疗炎症 (Nitta et al., 2007 and Rodriguez-Caso et al., 2003) Histidine decarboxylase (HDC) catalyzes histamine formation from histidine. Histamine is a bioactive amine acting as a neurotransmitter as well as a chemical mediator. Phenolic food components have been tested for their ability to inhibit recombinant human HDC. Epicatechin gallate (ECG) was found to be a potent inhibitor as it inhibited HDC activity in a competitive manner with Ki = 10 μ M against L-histidine. Epigallocatechin gallate (EGCG) showed time-dependent inhibition which disappeared under anaerobic conditions. It is probable that time-dependent inhibition could be due to the result of autoxidation of EGCG. The initial burst observed for EGCG suggests that EGCG itself is involved in HDC inhibition as observed for ECG. Our present results have shown that the tested food components can inhibit HDC activity. This inhibition likely affects histamine biosynthesis and possibly leads t 组氨酸脱羧酶(HDC)催化组氨酸的组胺的形成。组胺是一种生物活性胺作为一种神经递质以 及化学介质。酚醛食品组件测试的能力抑制人类HDC 重组。表儿茶素没食子酸盐(ECG)被 发现是一个强有力的抑制剂,抑制HDC 活动竞争的方式与Ki = 10 μ M 组氨酸。儿茶素没食 子酸盐(EGCG)显示时间抑制而在厌氧条件下就会消失。时间很可能是抑制可能是由于 EGCG 的自氧化的结果。EGCG 的初始破裂观察表明,EGCG 本身参与HDC 抑制作为心电 图观察。我们目前的结果表明,测试食品成分可以抑制HDC 活动。这种抑制作用可能影响组 胺生物合成并可能导致t Abstract Histamine, a bioactive amine, is formed from histidine through histidine decarboxylase (HDC). The agent that controls histamine synthesis, i.e. activity of HDC, is beneficial for controlling histamine-related physiological actions, such as inflammation. I