大叶桉炭疽病病原菌的鉴定及室内药剂筛选（行业资料）.docVIP

大叶桉炭疽病病原菌的鉴定及室内药剂筛选（行业资料） 文档信息 ： 文档作为关于“行业资料”中“医学资料”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文10418字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大叶桉炭疽病病原菌的鉴定及室内药剂筛选 1 1材料与方法 4 2结果与分析 6 3结论与讨论 9 文2：番茄黑斑病病原菌的鉴定及生物学特性研究 11 1材料与方法 11 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 大叶桉炭疽病病原菌的鉴定及室内药剂筛选（行业资料） 文1：大叶桉炭疽病病原菌的鉴定及室内药剂筛选 Identification of the pathogen causing Eucalyptus anthrax and laboratory screening of fungicides ZENG Xueying Abstract：We fit discovered a suspected disease named anthrax Eucalyptus robusta planting regio in Panzhihua， Sichuan， in 2014. We obtained the representative strain Z-D-07 with the methods of sampling， separation， single spore culture， as well as pathogenicity tests， and we also identified the species of Z-D-07 based on morphological and developmental systems rDNA?ITS sequences. The results showed that the strain Z-D-07 in morphological characteristics were cois?tent with Colletotrichum boninee. By using the fungal univeal prime ITS1 and ITS4， we amplified the internal tracribed spacer rDNA sequences of the strain Z-D-07， then after recyclying， cloning and sequencing the amplification products， a length of 597 bp gene fragments was obtained. The results of blast in Genebank， the analysis of fungal rDNA systemic development and phylogenetic tree showed that the relation between this fragment and the fungi of Colletotrichum boninee was closest， so morphological and molecular identification confirmed this fungal pathogen strain Z-D-07 as Colletotrichum boninee. Six kinds of common pesticides were used to study the control effects on Eucalyptus anthrax in the test， the results showed that 70% mancozeb presentd the optimum control efficiencies， and 80%thiram took second place。 Key words：Eucalyptus robusta Smith；anthrax；Colletotrichum boninee；phylogenetic tree； termiticides Author’s address： Sichuan Normal Univeity，Chengdu 610068，China 收稿日期：2015-01-07 修回日期：2015-03-29 基金项目：四川省科技支撑项目（2011YZGG-10） 作者简介：曾学英（