六微生物的生长及其控制.pptxVIP

Section A Determination of growth and reproduction （测定生长繁殖的方法） Determination of growth quantum（quantity）,suitable for all microbes. (测定生长量) Counting reproduction number, only suitable for unicelled bacteria and yeast, not for the filamentous moulds and actinomyces. (计繁殖数) ;A. Measuring of growth quantity（测生长量）;turbidimetry; B. Offspring counting ;; Colorless ( living) Yeast (methylene blue) Blue ( dead);The growth of filamentous microbe;2. Indirect ways (living cell counting) The methods are designed on the basis that living microbe can increase the turbidity while growing in liquid medium and can form colony on the solid medium. The most common used method is colony-counting. ;2.1 plate colony-counting methods(平板菌落计数法) The method is suitable for aerobic and anearobic microbes, every living cell will form a colony forming unit (菌落形成单位cfu), the total number of living cell in the sample can be calculated from cfu × dilution factor(稀释倍数). pour plate (浇注平板) spread plate (涂布平板);;2.2 anaerobic colony-counting method Hungate roll-tube technique (亨盖特滚管技术) is designed by famous American microbiologist R. E. Hungate in 1950. it’s a epoch-making invention in the development history of microbiology which made the isolation and study for the obligate anearobic possible. The method above needs complicated equipment and the skillful technique. Zhou deqing invented semi-solid media deep agar method(半固体深层琼脂法) which is easily manipulated.;Section B Rules of microbial growth Part A cell growth and synchronous growth Part B typical growth curve of unicelled microbe Part C continuous culture Part D high cell-density culture;Part A cell growth and synchronous growth ;Synchronous culture trying to keep a group of cells synchronous in cell growth and at the same stages of cell cycle , thus the individual biochemical chan